TABLE 1.

CT values from cells attached to stainless steel coupons and lysed under different boiling conditionsa

Boiling time (min)Expt no.bCT value after boiling coupons in different concentrations of SDS
0%0.1%1%2%
10112.94 ± 0.1616.17 ± 0.0317.07 ± 1.2816.20 ± 0.37
214.95 ± 0.3315.37 ± 0.4417.24 ± 1.20
30117.23 ± 1.6020.00 ± 0.7419.31 ± 1.5420.47 ± 0.91
215.95 ± 1.7019.41 ± 2.7119.65 ± 0.29
60117.80 ± 4.6821.39 ± 0.2619.17 ± 1.1617.02 ± 1.45
218.75 ± 0.6723.94 ± 0.1823.47 ± 0.48
  • a The CT value in each experiment is the mean of results from two coupons from which two real-time PCR cycles have been run.

  • b Individual growth experiments were run in duplicates.