TABLE 3.

Identification of PCB-metabolizing bacteria isolated from various plant species by 16S rRNA gene sequence analysis using RDP II

IsolatesNearest sequence match in RDP IIAssociation with plant speciesa
DesignationGenBank accession no.Strain or speciesGenBank accession no.ReferenceNo. of base pairs compared% SimilarityRoot zoneRhizosphere
Gram-positive High-G+C-content bacteria
    Arthrobacter subdivision
        OUCZ22 AY785732 Arthrobacter oxidans type strain X83408 24 1,27599.9 L
        OUCZ46 AY785738 Microbacterium oxydans type strain Y17227 43 1,204100.0A, BB, G, P
        OUCZ212 AY785751 Microbacterium sp. strain VKM Ac-2016 AB042081 Unpublished740100.0 P
    Mycobacterium subdivision
        OUCZ100 AY785746 Rhodococcus erythreus type strain X79289 39 1,29699.8A, (B), (G), L(A), (B), (G), L
        OUCZ108 AY785747 Rhodococcus erythreus type strain X79289 39 1,29699.8 A , (B), (G), L(A), (B), (G), L
        OUCZ211 AY785750 Rhodococcus erythropolis AF532870 49 1,296100.0 L
        OUCZ20 AY785731 Rhodococcus erythropolis isolate EK5 AJ237967 22 1,257100.0(A), L, (W)
        OUCZ91B AY785745 Rhodococcus ruber AF350248 Unpublished1,320100.0 B , G, W
        OUCZ16 AY785730 Rhodococcus sp. strain IFO16253 AB023374 Unpublished1,27499.3 A
        OUCZ35 AY785735 Rhodococcus sp. strain 871-AN040 AF420421 7 1,27599.9 P
        OUCZ26 AY785734 Rhodococcus sp. strain 871-AN040 AF420421 7 1,267100.0 A
        OUCZ44 AY785736 Rhodococcus sp. strain 871-AN040 AF420421 7 1,358100.0A, (L), WA, (B), G, W
        OUCZ58 AY785741 Rhodococcus sp. strain 871-AN040 AF420421 7 1,383100.0A, (L), WA, (B), (G), W
        OUCZ204 AY785749 Rhodococcus sp. strain 871-AN040 AF420421 7 1,37499.9 A
        OUCZ48 AY785739 Rhodococcus wratislaviensis type strain Z37138 Unpublished1,438100.0A, B, G, L, NR, P, W P
        OUCZ59 AY785742 Rhodococcus wratislaviensis type strain Z37138 Unpublished1,43499.9 A
        OUCZ45 AY785737 Williamsia murale type strain Y17384 21 1,44399.0 G
Low-G+C-content bacterium
    OUCZ63 AY785743 Bacillus sp. strain LMG 20240 AJ316310 16 1,444100.0 G
Gram-negative Gammaproteobacteria
    OUCZ70 AY785744 Luteibacter rhizovicina type strain LJ96T AJ580498 Unpublished1,35799.0W
    OUCZ118 AY785748 Pseudomonas fluorescens strain Biotype F AJ308306 Unpublished1,35499.9P
    OUCZ24 AY785733 Pseudomonas frederiksbergensis strain JAJ28 AJ249382 1 1,258100.0L, WL
    OUCZ52A AY785740 Pseudomonas sp. strain SMCC B0361 AF500621 51 1,34299.9G, W
  • a The data indicate that a bacterium with same the 16S rRNA gene sequence was isolated from the root zone or rhizosphere of other plant species at the site and sequenced. A, ash; B, birch; G, Grass-forb mixture; L, Black locust; NR, non-root-containing soil; P, pine; W, willow. Parentheses indicate that a bacterium with the same cell and colony morphology was isolated but not sequenced; thus, the identity is tentative. Underlining indicates the isolation source.