TABLE 3.

Primer/probe pair amplification efficiencies of different DNA targets

Target geneTemplatenaAmplification efficiencySlopey-InterceptR2Linear range (copies per μl DNA)
Bacterial 16S rRNApBAV1/16S51.90 ± 0.05−3.58 ± 0.1442.6 ± 1.0>0.9601.7 × 102-1.7 × 108
BAV1 DNA51.90 ± 0.07−3.41 ± 0.1242.5 ± 0.8>0.9851.2 × 102-1.2 × 108
pFL2/16S31.99 ± 0.06−3.34 ± 0.1442.7 ± 1.3>0.9901.8 × 101-1.8 × 108
GT DNA31.93 ± 0.02−3.51 ± 0.0541.4 ± 0.8>0.9264.6 × 102-4.6 × 106
2CP-C DNA31.97 ± 0.10−3.39 ± 0.2640.3 ± 2.0>0.9918.9 × 102-8.9 × 106
Dehalococcoides 16S rRNApBAV1/16S131.84 ± 0.07−3.80 ± 0.2243.3 ± 2.1>0.9851.7 × 101-1.7 × 108
BAV1 DNA61.78 ± 0.10−3.85 ± 0.3343.8 ± 2.7>0.9751.2 × 101-1.2 × 107
pFL2/16S31.93 ± 0.07−3.51 ± 0.2143.2 ± 1.8>0.9551.8 × 101-1.8 × 108
FL2 DNA81.85 ± 0.07−3.76 ± 0.2442.3 ± 1.8>0.9853.0 × 100-3.0 × 107
GT DNA51.97 ± 0.05−3.41 ± 0.1242.3 ± 0.8>0.9852.1 × 101-2.1 × 106
195 DNA31.96 ± 0.20−3.40 ± 0.4040.6 ± 2.0>0.9853.6 × 102-3.6 × 107
2CP-C DNA3NAb
bvcA genepBAV1/bvcA81.77 ± 0.04−4.05 ± 0.1841.8 ± 2.6>0.9751.7 × 101-1.7 × 108
BAV1 DNA41.86 ± 0.11−3.74 ± 0.3345.1 ± 2.8>0.9951.2 × 101-1.2 × 108
GT DNA3NA
FL2 DNA3NA
195 DNA3NA
2CP-C DNA3NA
vcrA geneBAV1 DNA3NA
pGT/vcrA82.00 ± 0.04−3.31 ± 0.0939.7 ± 0.7>0.9851.8 × 101-2.1 × 108
GT DNA52.00 ± 0.11−3.33 ± 0.2740.7 ± 2.6>0.9941.6 × 101-1.6 × 106
FL2 DNA3NA
195 DNA3NA
2CP-C DNA3NA
tceA geneBAV1 DNA3NA
GT DNA3NA
pFL2/tceA81.81 ± 0.11−3.89 ± 0.2245.1 ± 2.7>0.9951.3 × 101-1.3 × 108
FL2 DNA31.81 ± 0.06−3.89 ± 0.0247.3 ± 3.3>0.9756.0 × 102-6.0 × 107
195 DNA31.85 ± 0.1−3.76 ± 0.3143.5 ± 3.1>0.9853.6 × 102-3.6 × 107
2CP-C DNA3NA
  • a n indicates the number of replicates of independent dilution series.

  • b NA, no amplification.