TABLE 1.

List of strains, plasmids, cosmids, and vectors used

NameGenotype or descriptionaReference or source
Strains
    B. gladioli pv. agaricicola
        BG164Wild type; Cav+ Af+ Rifs Prot+18
        BG164RSpontaneous Rifr mutant of BG164; Cav+ Af+ Rifr Prot+This study
        BG4-12BG164R gspF::mini-Tn5KmlacZ2 Rifr Kmr Cav Af+ ProtThis study
        BG12-88BG164R gspK::mini-Tn5KmlacZ2 Rifr Kmr Cav Af+ ProtThis study
        BG12-147BG164R gspK::mini-Tn5KmlacZ2 Rifr Kmr Cav Af+ ProtThis study
        BG15-40BG164R gspE::mini-Tn5KmlacZ2 Rifr Kmr Cav Af+ ProtThis study
        BG15-87BG164R gspD::mini-Tn5KmlacZ2 Rifr Kmr Cav Af+ ProtThis study
        BG16-787BG164R gspE::mini-Tn5KmlacZ2 Rifr Kmr Cav Af+ ProtThis study
        BG4-12CosBG4-12 complemented with cosmid pCosGSP; Rifr Kanr Tetr Cav+This study
        BG12-88CosBG12-88 complemented with cosmid pCosGSP; Rifr Kanr Tetr Cav+This study
        BG15-40CosBG15-40 complemented with cosmid pCosGSP; Rifr Kanr Tetr Cav+This study
        BG-LAF3BG164R with pLAFR3; Rifr Tetr Cav+ Prot+This study
        BG4-12LAF3BG4-12 with pLAFR3; Rifr Kanr Tetr Cav ProtThis study
    E. coli
        S17-1 λpirthi pro hsdR hsdM+ ΔrecA RP4-2::TcMu Km::Tn732
        DH5αsupE44 ΔlacU169 (φ80lacZΔM15) hsdR17 thi-1 relA1 recA116
        HB101supE44 hsdS20 (rB mB) recA13 ara-14 rspL20 proA2 lacY1 galK2 xyl-5 myl-13
    P. aureofaciens
        PA147-2Wild type; Af+ Bfm+ Rifr Cmr4
        PAE639PA147-2; yeiJ::mini-Tn5KmlacZ2 Af+ Bfm Fla Mot Rifr KmrH. K. Mahanty
    P. cepacia B111Wild type; ChiA. L. J. Cole
    P. putidaWild type; ProtH. K. Mahanty
    S. entomophila A1MO2Derivative of A1 wild type; Ampr Chi+ Path+36
Plasmids and cosmids
    pUTZ2pUT containing mini-Tn5KmlacZ27
    pSPRC128.5-kb SalI fragment containing gspF::mini-Tn5KmlacZ2 from BG4-12 in pBluescript KS+; Apr KanrThis study
    pSPRC405.4-kb SalI fragment containing gspE::mini-Tn5KmlacZ2 from BG15-40 in pBluescript KS+; Apr KanrThis study
    pSPRC876.0-kb SalI fragment containing gspD::mini-Tn5KmlacZ2 from BG15-87 in pBluescript KS+; Apr KanrThis study
    pSPRC885.2-kb SalI fragment containing gspK::mini-Tn5KmlacZ2 from BG15-88 in pBluescript KS+; Apr KanrThis study
    pSPRC1475.3-kb SalI fragment containing gspK::mini-Tn5KmlacZ2 from BG12-147 in pBluescript KS+; Apr KanrThis study
    pSPRC7875.4-kb SalI fragment containing gspE::mini-Tn5KmlacZ2 from BG12-787 in pBluescript KS+; Apr KanrThis study
    pROBE1.5-kb NotI fragment containing the kanamycin gene from mini-Tn5KmlacZ2 blunt-end cloned into the EcoRV site of pBluescript KS−; AprS. R. Giddens
    pCosGSP23.4-kb Sau3AI partial fragment containing the GSP gene cluster from BG164R into pLAFR3This study
    pGSP ME H-H16.716.7-kb HindIII subclone from pCosGSP in pME6001; GenrThis study
    pGSP ME H-H22-kb HindIII subclone from pCosGSP in pME6001; GenrThis study
    pGSP ME H-E1.71.7-kb HindIII-EcoRI subclone from pCosGSP in pME6001; GenrThis study
    pGSP KS B-B2.22.2-kb BamHI subclone from pCosGSP in pBluescript KS+; AprThis study
    pGSP KS B-B7.27.2-kb BamHI subclone from pCosGSP in pBluescript KS+; AprThis study
Vectors
    pBluescriptColE1 ori lacZα/KS polylinker; T3/T7; Apr (KS, M13+)Stratagene
    pLAFR3pRK290 derivative; RP4(IncP-1) ori λcos; pUC9 multicloning site and lacZα Tcr33
  • a Cav, cavity disease; Prot, protease; Chi, chitinase; Bfm, biofilm; Af, antifungal.