TABLE 1.

Blast results of ORFs identified on the metagenome cosmid clones

ORFSize (aaa)Possible function and GenBank accession no.Microorganism% Identity (no. of similar aa/total no.)
pCosCE1
    ORF001271Hypothetical protein, NP_669416Yersinia pestis48 (123/256)
    estCE1389β-Lactamase class C, ZP_00486659Burkholderia pseudomallei51 (196/378)
    ORF003310Probable regulator, NP_103137.1Mesorhizobium loti42 (124/293)
    ORF004224Putative transcriptional regulator, tetR family, ZP_00235387Bacillus cereus40 (78/191)
    ORF005385Arabinose efflux permease, ZP_00136708Pseudomonas aeruginosa45 (171/380)
    ORF006379β-Lactamase class C, CAB69829.1Serratia marcescens77 (302/378)
pCosCS-A3
    ORF001359Aminoglycoside phosphotransferase, ZP_00579208.1Sphingopyxis alaskensis79 (280/353)
    ORF002253Oxidoreductase, NP_421870.1Caulobacter crescentus73 (186/254)
    ORF003265Short-chain dehydrogenase/reductase ZP_00579206.1Sphingopyxis alaskensis81 (216/265)
    estA3396β-Lactamase, ZP_00579205.1Sphingopyxis alaskensis59 (236/396)
    ORF005301Protein of unknown function, ZP_00577139.1Sphingopyxis alaskensis57 (158/277)
    ORF006171Similar to aspartyl protease, ZP_00577809.1Sphingopyxis alaskensis40 (49/120)
    ORF007153Putative protein disulfide isomerase, ZP_00375704.1Erythrobacter litoralis43 (58/133)
    ORF008108Hypothetical protein, ZP_00567441.1Frankiasp.28 (29/101)
  • a aa, amino acids.