TABLE 3.

Phylogenetic association and physiological characteristics of isolates obtained from the different pulse intervals

Phylum and isolateaNearest GenBank relative% SimilarityAccession no.Growth kinetics when incubated in batch culture before transfer for:Ectoaminopeptidase activity (nmol MUF h−1 ml−1 mg protein−1)
1 day3 days7 days14 days
Lag (h)μ (h−1)Lag (h)μ (h−1)Lag (h)μ (h−1)Lag (h)μ (h−1)
Bacteroidetes
    1 A-5bChryseobacterium sp.98AY27848400.5700.5410.4220.401,273
    1 A-9bChryseobacterium meningosepticum ATCC 3395899AJ70454300.5710.5330.3430.352,236
    1 C-3C. meningosepticum ATCC 3395899AJ70454300.4200.4030.2320.232,232
    3 A-6bC. meningosepticum ATCC 3395899AJ70454300.4800.4230.3620.412,952
    3 C-3bC. meningosepticum ATCC 3395899AJ70454300.4100.3530.2930.382,711
    7 C-2bC. meningosepticum ATCC 49470100AJ70454400.5710.5810.4100.482,590
    3 A-13bFlavobacterium aquatile94M6279700.5300.4130.3230.34764
    3 B-3bFlavobacterium sp. strain 3A595AF36875600.3600.3500.2100.19488
    7 A-6bFlavobacterium sp. strain 3A595AF36875600.3310.3210.4220.18536
    7 B-4bFlavobacterium frigoris94AJ60139300.3800.3610.2310.21330
    1 B-1b,cSphingobacterium multivorum99AB10073800.4900.4820.4030.351,095
    1 B-8bSphingobacterium sp. strain MG298AY55641700.5110.520.3350.331,127
    1 C-2Sphingobacterium sp. strain MG298AY55641700.4210.4320.3320.421,071
    3 A-3Sphingobacterium sp. strain MG298AY55641700.3310.3820.3320.281,074
    7 A-7Pedobacter africanus DSM 12126T98AJ43817100.4520.4230.3840.34210
    7 A-12P. africanus DSM 12126T99AJ43817110.6340.5650.3340.30299
    14 A-12P. africanus DSM 12126T98AJ43817100.1310.3330.2430.23416
    14 C-13Flexibacter sp. strain MDA 249595AY238335120.06110.07110.06120.061,006
Alphaproteobacteria
    7 A-14Sphingomonas sp. strain JSS-2699AF13129630.5820.3230.2740.20167
Betaproteobacteria
    1 C-10bPseudomonas huttiensis ATCC 14670T99AB02136600.3700.3930.2920.342,181
Gammaproteobacteria
    1 B-3Stenotrophomonas acidaminiphila99AF27307900.3810.4020.3230.37119
    3 B-1S. acidaminiphila99AF27307900.3000.2200.1800.3035
    3 B-2bStenotrophomonas maltophilia99AY44507900.4810.5120.3430.4345
    7 B-9S. acidaminiphila99AF27307900.5700.6530.5820.622,482
    7 B-2bS. acidaminiphila99AF27307900.4210.4720.5130.5622
    14 A-9bS. acidaminiphila99AF27307900.3010.2930.2230.2119
    3 C-10bBlackwater bioreactor bacterium BW399AF39416800.4900.3520.2550.2343
    7 B-3bBlackwater bioreactor bacterium BW399AF39416800.3800.3830.3020.2832
    14 B-1bBlackwater bioreactor bacterium BW399AF39416820.4810.4520.3930.2642
    14 A-3bBlackwater bioreactor bacterium BW399AF39416800.3510.3310.4330.2439
Actinobacteria
    1B-14Arthrobacter sp. strain R33S97AY572475NDdND20.2220.2340.1942
    7 A-1Arthrobacter mysorens DSM 12798T98AJ61748200.4510.4630.2740.23126
  • a Isolates are coded by the pulse interval from which they were isolated (1, 3, 7, or 14), followed by the replicate (A, B, or C) and isolate number

  • b Mobility of PCR amplicon on DGGE matched that of a member of the mixed community.

  • c Single isolates with two slightly different 16S rRNA gene sequences. The 1B1 second sequence matched 98% to Sphingobacterium sp. strain MG2 (accession no. AY556417), and the 3A3 second sequence matched 99% to Sphingobacterium multivorum (accession no. AB100738).

  • d ND, not determined.