TABLE 2.

Characteristics of mutants having altered phenotypes

MutantMutated geneameanw for mutant grown in:Gene product
TYVMMTY+SDSTY+NaCl
Cluster 1 (highly competitive under most of the conditions tested)
    2-2ESMb216330.390.550.670.88PaaG enoyl-coenzyme A hydratase protein
    3-5ASMb200370.900.790.650.71AroE2 shikimate 5-dehydrogenase protein
    3-6DSMc030320.510.290.830.81FlgI flagellar P-ring precursor transmembrane protein
Cluster 2 (highly competitive in TY medium and VMM under nonstress conditions)
    1-3GpSymB.5439711.460.77−0.010.12Intergenic
    1-5GSMa06211.020.790.070.12FixI2 E1-E2-type cation ATPase
    3-12BSMb204760.920.61−0.090.14Putative ABC transporter periplasmic dipeptide-binding protein
Cluster 3 (impaired growth in VMM)
    1-11CSMc04346−0.33−1.08−0.11−0.04IlvC ketol-acid reductoisomerase protein
    2-9ASMc028990.10−0.830.260.04PheA prephenate dehydratase protein
    3-1CSMc01842−0.20−0.99−0.150.08Putative methyltransferase transcription regulator protein
    4-12CSMc01053−0.32−1.35−0.08−0.01CysG siroheme synthase protein
    4-1HSMc03776−0.65−1.230.210.01ProB1 glutamate 5-kinase protein
Cluster 4 (highly competitive in stress conditions)
    2-11BSMc01881−0.50−0.472.092.03PanB 3-methyl-2-oxobutanoate hydromethyltransferase protein
    2-5HSMc03164−0.18−0.080.770.43XylB xylulose kinase protein, putative
    2-7HpSymB.964350.280.110.820.43Intergenic
    3-10HSMb20360−0.36−0.221.671.50Hypothetical protein, putative protease subunit of ATP-dependent Clp protease
    3-11BpSymB.10038460.190.431.951.85Intergenic
    3-5BSMb209310.120.210.940.76Putative sugar uptake ABC transporter periplasmic solute-binding protein precursor
Cluster 5 (impaired growth under all conditions tested; strongly impaired growth in VMM)
    2-9ESMc03782−0.40−1.01−0.37−0.52Hypothetical signal peptide protein
    3-7DSMc01174−0.58−0.77−0.40−0.37CysK2 cysteine synthase A protein
Cluster 6 (low competitiveness under normal conditions and slightly increased competitiveness under stress conditions)
    3-11DSMb20377−0.67−0.900.460.46Putative translation initiation inhibitor protein
    3-12DSMc00334−1.26−0.980.790.51Cmk cytidylate kinase protein
Cluster 7 (low competitiveness under normal conditions but not disadvantaged in stress conditions)
    3-12CSMc00808−0.76−0.350.210.15ChrA chromate transport protein
    4-10CSMa0091−0.75−0.420.130.13Conserved hypothetical protein
    4-11CSMc02597−0.72−0.50−0.03−0.13SodC superoxide dismutase Cu-Zn precursor transmembrane protein
    4-12DSMa0070−0.75−0.460.130.08ABC transporter permease
Cluster 8 (impaired growth in TY medium and partially impaired growth under other conditions)
    2-1HSMc01219−0.64−0.35−0.74−0.19Lipopolysaccharide core biosynthesis mannosyltransferase LpsB
    3-11HSMc01700−0.72−0.31−0.26−0.24Peptidyl-prolyl cis-trans isomerase A (PpiA)
    3-8CSMc02050−0.74−0.70−0.54−0.49Trigger factor protein Tig, probable
    4-12BSMc02144−1.17−0.65−0.70−0.62PstC phosphate transport system permease ABC transporter protein
  • a For mutants carrying a transposon insertion in an intergenic region the exact position of insertion in the replicon is given.