TABLE 1.

Details concerning the oligonucleotide probes used in the present study for FISH

Probe or oligonucleotideaTarget (position)bSequence (5′→3′)Applied stringency (% formamide)Reference
EUB338Bacterial 16S rRNA (338-355) GCTGCCTCCCGTAGGAGT 10 2
CREM62 L. lactis subsp. cremoris 16S rRNA (62-80) CCAATCTTCATCGCTCAA 10This study
H44 L. lactis subsp. cremoris 16S rRNA (44-61) CTTGCATGTATTAGGCAC 10This study
H81 L. lactis subsp. cremoris 16S rRNA (81-99) TTCAAATYGGTGCAAGCA 10This study
LAC62 L. lactis subsp. lactis 16S rRNA (62-80) CCAACCTTCAGCGCTCAA 10This study
LEUC1026 Leuconostoc spp. 16S rRNA (1026-1043) CACTTTGTCTCCGAAGAG 10This study
H1009 Leuconostoc spp. 16S rRNA (1009-1025) AACACTTCTATCTCTAAA 10This study
H1044 Leuconostoc spp. 16S rRNA (1044-1061) CGACCATGCACCACCTGT 10This study
NON338Negative control ACTCCTACGGGAGGCAGC 10 31
  • a Helper oligonucleotides are indicated by the prefix H.

  • b Base pair positions are based on E. coli numbering (7).