TABLE 4.

Recovery of 2 ×105 plasmid DNA copies spiked into human and canine fecal dilutions

Fecal sourceSample fecal concn (mg/liter)Measured no. of recovered DNA copies% PCR amplification of plasmid relative to controla
Human5,0002.6 (±0.02) × 10591
1,0003.3 (±1.0) × 105116
2002.7 (±0.2) × 10596
403.7 (±0.8) × 105124 (±29)
82.9 (±0.1) × 10599 (±4.5)
1.62.6 (±0.5) × 10589 (±16)
0.322.9 (±0.4) × 10599 (±13)
Canine10,0002.2 (±0.4) × 10575 (±15)
2,0003.6 (±0.8) × 105123 (±26)
4002.9 (±0.1) × 105100 (±4.1)
802.7 (±0.2) × 10591 (±8.3)
162.3 (±0.6) × 10578 (±20)
3.22.4 (±0.4) × 10582 (±14)
0.642.6 (±0.4) × 10588 (±15)
Control02.8 (±0.1) × 105100
  • a Percent PCR amplification = (measured no. of copies in the presence of feces/measured no. of copies in the control) × 100.