Table 2

Results of fecal and tissue panels for culturing Mycobacterium avium subsp. paratuberculosisa

Sample type and testResult for:
Bison ABison BBison CBison DUSDA positive controlUSDA negative control
Fecal
    TREK+++
    TREK-PD+NI+
    TREK-DC+NI+
    TREK-NENI
    TREK-FBS+NI+
    TREK-pyr+NI+
    HEY+NI+
    HEY-PD+NI+
    HEY-DC+NI+
Tissue
    TREK++NINI
    TREK-PDNI+NINI
    TREK-FBSNINININI
    TREK-pyrNINININI
    HEY+NINI
    LJ+NINI
    LJ-pyr+NINI
    7H11NINI
    7H11-pyrNINI
  • a A culture variation was recorded as positive if at least one of the replicate slants had growth. Bison A and B are wood bison from the Mackenzie bison herd for which samples were positive on direct fecal PCR and from which tissues were also available. Bison C is a commercial plains bison for which samples were positive on direct fecal PCR and which is known to be culture positive according to TREK ESP. Bison D is a wood bison from a wildlife preserve that was known to be fecal culture positive according to TREK ESP. Positive and negative control feces are from the NVSL 2011 direct PCR proficiency panel. NI, not included; PD, PANTA decontamination; DC, double centrifugation; NE, no egg yolk; FBS, fetal bovine serum; pyr, pyruvate; HEY, Herrold's egg yolk; LJ, Lowenstein-Jensen; 7H11, Middlebrook 7H11.