TABLE 3.

Percentages of product yield from PCR with plasmids containing zero to seven mismatches to the primers and probe in a real-time PCR assay

PlasmidAssayNo. of mismatches% Product yielda
TNHu1-4HuBac0100
TNBo1-5BoBac0100
TNCa2-1HuBac133.5 ± 18
ANAv1-13HuBac37.6 ± 5.8
TNCa1-1HuBac43.2 ± 1.5
TNAv1-7HuBac64.2 × 10−3 ± 2.0 × 10−3
TNAv1-13BoBac69.7 × 10−3 ± 2.6 × 10−3
TNBo1-5HuBac72.1 × 10−5 ± 1.9 × 10−5
  • a Means and standard deviations of the percent product yield for each plasmid concentration ranging from 25 to 2.5×107 copies. Percent product yield for each plasmid concentration = (copies calculated from 0 mismatch standard curve/expected copies)×100.