TABLE 2.

Quantitative FISH analysis and total cell counts of the four river sedimentsa

ProbeEBHWFL
Normalized microarray signal (±SD)b% Hybridized cells of all DAPI-stained objectsNormalized microarray signal (±SD)b% Hybridized cells of all DAPI-stained objectsNormalized microarray signal (±SD)b% Hybridized cells of all DAPI-stained objectsNormalized microarray signal (±SD)b% Hybridized cells of all DAPI-stained objects
EUB 33823.0 (±2.6)7113.6 (±5.1)5710.0 (±0.4)532.3 (±0.2)35
EUB II2.5 (±0.1)6 ND 3 2.3 (±0.1)6ND<1
EUB III6.6 (±0.4)2012.1 (±3.0)224.4 (±0.7)173.1 (±1.2)6
EURY514 ND 1 ND 1 4.7 (±0.3)1ND<1
EURY499ND<12.9 (±0.6)2ND<1NDND
EURY496ND<1 ND 2 ND<1NDND
EUK1195 2.1 (±0.1) ND NDNDNDNDNDND
BLS1295 ND 2 4.4 (±0.9)42.9 (±0.3)5ND<1
BTWO23a8.1 (±0.5)82.5 (±0.5)42.7 (±0.2)4ND<1
DSS6583.4 (±0.6)43.5 (±0.8)6 ND 3 NDND
Am445 2.1 (±0.2) <1 3.7 (±0.9)3 ND 1 NDND
CF319a+bNDNDNDNDNDNDNDND
PLA46NDNDNDNDNDNDNDND
SRB385, SRB385DbNDNDND<1ND<1NDND
LGCNDNDND<1ND<1NDND
LGC354A-CNDNDNDNDNDNDNDND
  • a Total cell counts were as follows: for EB, 4.0 × 108; for HW, 1.9 × 109; for FL, 1.3 × 109; DÖ, 6.0 × 107. Values in boldface are discussed in the text. Underlined values represent relative cellular abundances of 1% or higher in combination with absent microarray signals (false negatives). Since for the same probes, microarray signals were found for higher abundances, the absent signals are assumed to reflect a probe-specific detection limit of the format. All other values (not in boldface or underlined) represent matches of microarray signals and relative cellular abundances of 2% or higher as well as matches of absent microarray signals and relative cellular abundances well below 1% (indicated by <1 or ND, respectively).

  • b According to the results shown in Fig. 3.