TABLE 2.

Primers and probes used for quantitative real-time PCR

Primer or probeSequence
Primers
    gusA-1257F5′-AGGTGCACGGGAATATTTCG-3′
    gusA-1317R5′-ACGCGTCGGGTCGAGTT-3′
    nifA1-933F5′-CGGCCGGTTCGAATTG-3′
    nifA1-992R5′-GAAATCTCGCCGATTTCATCA-3′
    nifA2-122F5′-TCCCTATTGTGCCGTTGCA-3′
    nifA2-185R5′-GCGGTGAGCGCTATCGA-3′
    nifH-602F5′-CCGCCAGACTCAATTCCAA-3′
    nifH-662R5′-GCGTGCTGGACGATGTT-3′
    acdS-299F5′-GCTGGGTTCCACATGAGGAT-3′
    acdS-365R5′-CCCAAGATGCGGCTCAAG-3′
TaqMan probes
    gusA-1279TM5′-CCACTGGCGGAAGCAACGCG-3′
    nifA1-950TM5′-CGAATGGCGGAACCCTGCTGC-3′
    nifA2-143TM5′-AGAGGGCGCCGGACATCTTTGAAAC-3′
    nifH-625TM5′-ATCCACTTCGTGCCGCGC-3′
    acdS-323TM5′-TCTACGACCGGGTCGGCAACATTC-3′