TABLE 1.

Plasmid characteristics and construction

PlasmidResistanceConstruction and/or propertiesReference
KS+ΔH3AppBluescript KS (+) deleted for HindIII site in MCSThis paper
pOSV501ApCASS1 in KS+ΔH3 digested with EcoRVThis paper
pOSV502ApCASS2 in KS+ΔH3 digested with EcoRVThis paper
pOSV503ApCASS3 in KS+ΔH3 digested with EcoRVThis paper
att1ΩAbr+/−aAp AbpOSV501 + ΩAbr in HindIII siteThis paper
att2ΩAbr+/−aAp AbpOSV502 + ΩAbr in HindIII siteThis paper
att3ΩAbr+/−aAp AbpOSV503 + ΩAbr in HindIII siteThis paper
pWHM3Ap TsReplicative shuttle vector for E. coli and Streptomyces 26
pWHM3HygAp HmpWHM3, tsr replaced by hygThis paper
pOSV510Ap Hm prap-aph in pWHM3HygThis paper
pSET152GnIntegrative plasmid in Streptomyces 1
pOSV511Gn Hm att1Ωhyg in EcoRV site of pSET152This paper
pOSint3Ap trcp-xis-int in pTRC99A 18
pOSV507Ap Ts ermE *p-xis-int in pIJ903This paper
pOSV508Ap Ts trcp-xis-int in pWHM3This paper
pOS49.99Ap orf1-2-3-4 in pUC19Unpublished
pOSV512Ap Hm orf1-2-orf3::att1Ωhyg-orf4 in pOS49.99This paper
pOJ260GnIntegrative plasmid in Streptomyces 1
pOSV513Gn Hm Ap orf1-2-orf3::att1Ωhyg-orf4 in pOJ260This paper
pOS49.52Ap Ts ermE *p-orf3 in pIJ903Unpublished
pOS527Ap Ts prap in pIJ487 22
  • a Abr, antibiotic resistance gene aac or hyg; +, resistance gene transcribed in the attL-to-attR orientation; −, resistance gene transcribed in the opposite orientation.