TABLE 1.

Strains used in this study

Species or strainDescriptionSource
Mycobacterium species
    M. avium subsp. paratuberculosisStrain 6783 (DSM 44135)Laboratory reference strain (clinical isolate)
    M. avium subsp. aviumDSM 44156/ATCC 25291TGerman Collection of Microorganisms and Cell Cultures (DSMZ)
    Two M. avium subsp. hominissuis clinical isolatesClinical isolatesI. Moser, Friedrich-Loeffler-Institut, Jena, Germany
    Six M. avium subsp. paratuberculosis field isolatesEU reference panel no. 25 and 30 (type I strains) and no. 4, 6, 40, and 49 (type II strains)K. Stevenson, Moredun Research Institute, Penicuik, Scotland
    M. fortuitumDSM 46621I. Moser, Friedrich-Loeffler-Institut, Jena, Germany
    M. diernhoferiDSM 43524I. Moser, Friedrich-Loeffler-Institut, Jena, Germany
    M. vaccaeField isolateI. Moser, Friedrich-Loeffler-Institut, Jena, Germany
    M. flavescensDSM 43991I. Moser, Friedrich-Loeffler-Institut, Jena, Germany
    M. gastriField isolateI. Moser, Friedrich-Loeffler-Institut, Jena, Germany
    M. gordonaeDSM 43212I. Moser, Friedrich-Loeffler-Institut, Jena, Germany
    M. malmoenseDSM 44163I. Moser, Friedrich-Loeffler-Institut, Jena, Germany
E. coli strain
    TOP10 F′F′ mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara leu)7697 galU galK rpsL StrrendA1 nupGTOPO TA cloning kit (Invitrogen, Groningen, The Netherlands)