TABLE 1.

Bacterial strains and plasmids used in this studya

Strain(s) or plasmid(s)Relevant characteristicsReference or source
E. coli strain DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK mK) phoA supE44 λ thi-1 gyrA96 relA1Invitrogen
Acinetobacter baylyi strains
    ADP1Naturally transformable isolate27
    ADP197recA100::Tn5 (Kmr)20
    ADP6146pcaH18; 1-bp insertion A504+A; ΔcatD101::Kmr18
    ADP6209ΔpcaH20; 1-bp deletion ΔΑ621; ΔcatD101::Kmr18
    ADP6407pcaH12; 1-bp substitution C304T; ΔcatD101::Kmr18
    ADP7021ΔmutS6::Ω(Str/Spr)47
    ADP7700 to ADP7768apcaG variantsThis study
    ADP7784pcaG7718 ΔrecG1::sacB-KmrThis study
    ADP7785pcaG7718 ΔrecG2; 1.8-kb deletion in recG Δ(G138-T1937)This study
    ADP7786pcaG7718 recA100::Tn5 (Kmr)This study
    ADP7787pcaG7718, ΔmutS6::Ω(Str/Spr)This study
    ADP7788pcaG7718; recG repairedThis study
    ADP8534ΔpcaHG::sacB-Kmr; 1.0-kb deletion in pcaHGA. Buchan
Plasmids
    pGEM-5ZfApr; cloning vectorPromega
    pRMJ1Apr; sacB-Kmr cassette-bearing plasmid25
    pUC18Apr; cloning vectorNEB
    pZR2Apr; 2.4-kb HindIII fragment containing pcaKCHG in pUC1818
    pZR7700pcaG7700; 1-bp insertion C237+T; nonsense mutation and frameshift in pcaGThis study
    pZR7701pcaG7701; 1-bp insertion C237+T; 1-bp substitution T231A; template for all pcaG variantsThis study
    pZR7702 to pZR7768apZR7701-derived pcaG variantsThis study
    pZR7780ADP1 3.0-kb insert of recG into pGEM-5ZfThis study
    pZR7781pZR7780 recG Δ(G138-T1937)This study
    pZR7782pZR7780 recG::BamHI (T1067G)This study
    pZR7783pZR7780 recGΔ::sacB-KmrThis study
  • a pcaG variants are characterized by their repeat lengths and distances between homologous base pairs; more-detailed descriptions of their structures are given in Table S2 in the supplemental material.