TABLE 2.

Validation of Roseburia probes

Control strainaHybridizationb with:
Rrec584Rint623 + helper
Streptococcus gordonii DL-1
Streptococcus bovis 26R
Streptococcus mutans DSM 20523
Enterococcus faecalis JH2-2
Enterococcus faecium DSM 20477
Faecalibacterium prausnitzii A2-165
Faecalibacterium prausnitzii (MMB)
Bifidobacterium adolescentis L2-32
Bifidobacterium longum NCIMB 8809
Bifidobacterium infantis DSM 20088
Bifidobacterium breve MMB 3035
Eubacterium hallii L2-7
Coprococcus-like strain L2-50
Eubacterium ventriosum DSM 3988
Eubacterium eligens (MMB)
Eubacterium siraeum DSM 3996
Anaerostipes caccae DSM 14662T
Bacteroides vulgatus DSM 1447T
Bacteroides thetaiotaomicron DSM 2079T
Bacteroides distasonis DSM 20701T
Bacteroides fragilis DSM 2151T
Eubacterium cylindroides T2-87
Eubacterium cylindroides MMB 3291
Collinsella aerofaciens DSM 3979
Lactococcus lactis MG1363
Lactococcus lactis DSM 20069
Lactobacillus acidophilus A274
Megasphaera elsdenii ATCC 25940R
Veillonella parvula DSM 2008
Acidaminococcus fermentans (MMB)
Escherichia coli JM109
Escherichia coli ATCC 25922
Ruminococcus albus SY3R
Ruminococcus flavefaciens 17R
Ruminococcus flavefaciens ATCC 19208R
Ruminococcus bromii L2-63
Desulfovibrio piger DSM 749
Clostridium acetobutylicum DSM 792
Clostridium barkeri MMB 3355
Clostridium polysaccharolyticum DSM 1801R
Clostridium nexile DSM 1787
Clostridium aminovalericum DSM 1283S
Clostridium butyricum MMB 3316
Methanobrevibacter smithii MS1
Eubacterium rectale A1-86++
Roseburia inulinivorans DSM 16841T+
Roseburia hominis DSM 16839T++
Roseburia intestinalis DSM 14610T++
  • a All cultures were of human origin except of those designated R (rumen) and S (sewage). The strains are available from the Deutche Sammlung von Microorganismen und Zellkulturen (DSMZ) (Braunschweig, Germany), American Type Culture Collection (ATCC) (Rockville, Maryland), or Laboratory for Medical Microbiology (MMB) (Groningen, The Netherlands) or otherwise are held in culture at the Rowett Research Institute.

  • b +, positive hybridization; −, no signal.