TABLE 3.

Constructed primer pairs used in this study

GroupTarget gene (synonym)PCR product size (bp)Annealing temp (°C)PrimerSequenceProduct
1STM309842365STM3098-f25′-TTTGGCGGCGCAGGCGATTCPutative transcriptional regulator
STM3098-r25′-GCCTCCGCCTCATCAATCCG
STM444425365STM4444-f5′-ATGCCGACTGGTCGTTCCCGPutative inner membrane protein
STM4444-r5′-CCCACGCCGGTCTGAATTGC
2STM034924965STM0349-f5′-TCGTCGGCTTGGCTTTAACCPutative outer membrane lipoprotein
STM0349-r5′-CTGCACGCTGGGTTAACAGG
STM403031065STM4030-f5′-AAACCGTCCCACTGATGGGGPutative cytoplasmic protein
STM4031-r5′-CGTTAGTGCTCTGCGGCCAT
3STM155018767STM1550-f5′-AGCTAAGGGAACGGCTTGAAPutative cytoplasmic protein
STM1550-r5′-CGTGTCATTTTGTAGACGGC
STM223517765STM2235-f5′-TGCAGTCAGTGGCAATAACGPutative phage protein
STM2235-r5′-CGTCACCTTTAGCCATCCCA
STM263011565STM2630-f5′-CTGCCGCAAATCCATTGATGHypothetical protein
STM2630-r5′-GTATTCAGCGCACTGCCTGG
STM274414165STM2744-f5′-CCGAAAGCGGCAACGTGCGTPutative cytoplasmic protein
STM2744-r5′-CCGCAGCATCGAAGACCACC
STM275220365STM2752-f5′-TTATTCCTCCCGGTCCCGGCPutative glucitol-specific PTS enzyme III
STM2752-r5′-CCCGGCGCAGTTAATCACCA
STM275518765STM2755-f5′-AGCTGCTTTTCGACGCCGGGPutative hexulose 6 phosphate synthase
STM2755-r5′-ACCGCCAGCATATCTGCCCC
STM420331665STM4203-f5′-CTGCCTTGCAACGTCCTGAAPutative phage baseplate protein
STM4203-r5′-CGCCATAACACCTCCGTTGA
STM421410965STM4214-f5′-ACGCTCGCCGACGGTCAGGAPutative cytoplasmic protein
STM4214-r5′-CTGGCACCAGGTGACGGCGG
STM449731063STM4497-f5′-AACAACGGCTCCGGTAATGAPutative cytoplasmic protein
STM4497-r35′-TGACAAACTCTTGATTCTGA
STM457115465STM4571-f5′-TTTGTGCAGGCCTCAGCGGGPutative outer membrane protein
STM4571-r5′-GGGCACTGTCATTGGGAGCA
4STM245327065STM2453-f5′-TTGTATGCCCTGCGTCCAGGPutative cytoplasmic protein
STM2453-r5′-GCTTCCTCCTGCCATCCGGA
STM262428465STM2624-f5′-CTGGTGAAAGAGCAGGGGCGHypothetical protein
STM2624-r5′-GCTCCCCCCTTGTTGATGCT
5STM126942565STM1269-f5′-GTGCAGCACCACTTTTGCCGPutative chorismate mutase
STM1269-r5′-GCGCTCTCAGCCACACCATA
STM127728965STM1277-f5′-AAGCGCGTCTATTTCCCGGCPutative cytoplasmic protein
STM1277-r5′-GGCGAGTATCTTTAGCGGCG
6STM053828865STM0538-f5′-TCTCTTCCACAGTCCCCGCTPutative outer membrane protein
STM0538-r5′-CTGTCGCCGCTGTTTAGCCC
STM353223365STM3532-f5′-TCCGCCAGTTCCGACCATTGPutative dihydrodipicolinate synthetase
STM3532-r5′-CCTGCGTGCTGGTGCTGCTA
7STM450931265STM4509-f5′-TGGCGTTCCGTCCTTGTCAGPutative cytoplasmic protein
STM4509-r5′-TTGCGCCCTTATCACGACGG
8STM030515565STM0305-f5′-CGGAAACAGGACGGGGCTGTPutative cytoplasmic protein
STM0305-r5′-CCGAAGGCGCAATGGAGGAT
STM140818765STM1408-f5′-TCCTCTGCAGAACCGAGCCAType III secretion system apparatus protein
STM1408-r5′-TAAGCGCTTGCGATGCTGCG
STM185911565STM1859-f5′-AACACGATGCCATTTTCAATPutative cytoplasmic protein
STM1859-r5′-TTGAGGTCAGTGTGCAATTC
STM205634265STM2056-f5′-TGATGTTTATCGGCCCCAGCPropanediol utilization protein
STM2056-r5′-CCAGCCTGCGTAAGCCACTC
STM369026865STM3690-f5′-GAAGTCGTTGGCCGCGTTGAPutative inner membrane lipoprotein
STM3690-r5′-GGAGTTGTTTCCAGCGAGGC
STM405713765STM4057-f5′-GGTGGCCTCGATGATTCCCGPutative inner membrane protein
STM4057-r5′-CCCACTTGTAGCGAGCGCCG
STM407116765STM4071-f5′-AAGCGGTGAAGTGTGCCTGTPutative mannose-6-phosphate isomerase
STM4071-r5′-CGGGGTGGCTGTCATTTTCC
STM431722065STM4317-f5′-GCGAAACCCTGAACCTGCGTHypothetical protein
STM4317-r5′-CGCAGTGCGGCATTAGGTGA
STM445721565STM4457-f5′-AGCAACAGCACGCTCCGTCGPutative transposase
STM4457-r5′-GATATGCGACGAAAGCGGCG
9STM033923265STM0339-f5′-GCCCTACCCGCCACAGCATCPutative fimbrial chaperone
STM0339-r5′-CCTGGCCTGCTTTGGGTTGA
10STM100616565STM1006-f5′-TCTGATTGCGGTTACCGGGCExcisionase
STM1006-r5′-TGCGCCTCGATCCACTGATC
11STM375216565STM3752-f5′-CGGCTTGGCGTATACAGCGAPutative cytoplasmic protein
STM3752-r5′-GCCTCCCTCCAGATACACGG
12STM028732865STM0287-f5′-CGTATTTGCCTGGGGCGGAAPutative periplasmic protein
STM0287-r5′-CGCCAGCTTCTGATCCCGTA
13STM243410365STM2434-f5′-AGATATCTGCGTGGCGCGAGPutative cytoplasmic protein
STM2434-r5′-ATCCGGGCCACTCTCCAGCA
14STM459657365STM4596-f5′-ATGAAGCAGTTAAACGGCGGPutative inner membrane protein
STM4596-r5′-GCTGCGTGAAAGCCCGGTTC
15STM2955S18665STM2955-f5′-CTTGGCGATGAACTGCGCGAPutative transcriptional regulator
STM2955-r5′-CTTTTCCCAGGCCTGCGGCT
16STM040917065STM0409-f5′-TCGGGAAACCATGGATGGGGHypothetical protein
STM0409-r5′-CACCGGCAAGGACGACACGT