TABLE 1.

Primer sequences developed for real-time PCR of type A and B V. vulnificus

PrimerSequencePositionaAmplicon size (bp)Type amplified
VvAF1bCATGATAGCTTCGGCTCAA171-190285A
VvAR1cCAGCACTCCTTCCACCATCAC435-455
VvBF1bGCCTACGGGCCAAAGAGG177-194841B
VvBR1cGTCGCCTCTGCGTCCAC1001-1017
  • a Position refers to the nucleotide numbers for the 16S rRNA sequences of X76333 (type A) and X76334 (type B) in GenBank.

  • b Primer derived from M. Vickery et al (42).

  • c Primer derived from this study.