TABLE 1.

MLST and wsp loci and primer features

Cluster categoryaLocus code (wMel)GeneProductPrimerGene length (bp)bAmplified nucleotide range (bp)bMLST fragment size (bp)
DesignationSequence (5′-3′)
α-ProteobacteriaWD_0146gatBGlutamyl-tRNA(Gln) amidotransferase, subunit BgatB_F1GAKTTAAAYCGYGCAGGBGTT1,425421-891369
gatB_R1TGGYAAYTCRGGYAAAGATGA
Escherichia coliWD_0301coxACytochrome c oxidase, subunit IcoxA_F1TTGGRGCRATYAACTTTATAG1,551491-977402
coxA_R1CTAAAGACTTTKACRCCAGT
Escherichia coliWD_0484hcpAConserved hypothetical proteinhcpA_F1GAAATARCAGTTGCTGCAAA74191-605444
hcpA_R1GAAAGTYRAGCAAGYTCTG
Escherichia coliWD_0723ftsZCell division proteinftsZ_F1ATYATGGARCATATAAARGATAG1,197274-798435
ftsZ_R1TCRAGYAATGGATTRGATAT
RickettsialesWD_1238fbpAFructose-bisphosphate aldolasefbpA_F1GCTGCTCCRCTTGGYWTGAT900241-749429
fbpA_R1CCRCCAGARAAAAYYACTATTC
WolbachiaWD_1063wspOuter surface proteinwsp_F1GTCCAATARSTGATGARGAAAC71485-688546
wsp_R1CYGCACCAAYAGYRCTRTAAA
  • a Clustering categories indicating the level of protein conservation.

  • b With respect to the wMel genome.