TABLE 1.

Primers used in this study

PrimerTarget genePredicted target groupSequence (5′ to 3′)aAnnealing temp (°C)Reference
A8f16SArchaeaTCC GGT TGA TCC TGC C55 or 59 62
A1492r16SArchaeaGGC TAC CTT GTT ACG ACT T55 62
A21f16SArchaeaTTC CGG TTG ATC CYG CCG GA64 8
A915r16SArchaeaGTG CTC CCC CGC CAA TTC CT64 8
Ee1MS932r16SANME-2AGC TCC ACC CGT TGT AGT59 5
B8f16SBacteriaAGR GTT TGA TCC TGG CTC AG50 or 55 62
B1492r16SBacteriaCGG CTA CCT TGT TAC GAC TT50 or 55 62
dsr1f dsrAB Sulfate reducersACS CAY TGG AAG CAC G54 65
dsr4f dsrAB Sulfate reducersGTG TAG CAG TTA CCG CA54 65
1F1 dsrAB Sulfate reducersCAG GAY GAR CTK CAC CG48 10
1RI dsrAB Sulfate reducersCCC TGG GTR TGR AYR AT48 10
MCRf mcrA Methanogens/ANMEsTAY GAY CAR ATH TGG YT50 or 55 55
MCRr mcrA Methanogens/ANMEsACR TTC ATN GCR TAR TT50 or 55 55
ME1 mcrA Methanogens/ANMEsGCM ATG CAR ATH GGW ATG TC50 or 55 16
ME2 mcrA Methanogens/ANMEsTCA TKG CRT AGT TDG GRT AGT50 or 55 16
ANME-2a-647r16SANME-2aTCT TCC GGT CCC AAG CCT45 to 64 29
ANME-2c-622r16SANME-2cCCC TTC GCA GTC TGA TTG46 to 64 29
ANME-2c-760r16SANME-2cCGC CCC CAG CTT TCG TCC47 to 64 29
  • a Y, C/T; R, A/G; M, A/C; W, A/T; K, G/T; H, A/C/T; D, A/G/T.