TABLE 2.

Intracellular accumulation of full-length fusion proteins and unfused scFv/scTCR and GFP

ScFv/scTCR classUnfusedFused
Secretion level (μg/liter)Intracellular (μg/liter)aIntracellular/ secretionFusion typeSecretion level (μg/liter)Intracellular (μg/liter)aIntracellular/ secretionRelative fluorescence/moleculeb
IntracellularSecreted
hAb34,800 ± 2007,700 ± 1,6001.6 ± 0.3I1,300 ± 50015,000 ± 1,00011 ± 0.81.00 ± 0.021.00 ± 0.01
II4,000 ± 30011,000 ± 2,9002.6 ± 0.71.35 ± 0.091.57 ± 0.23
III1,800 ± 10015,000 ± 8008.4 ± 0.52.20 ± 0.042.19 ± 0.19
hAb781 ± 125,400 ± 80067 ± 10I270 ± 10015,000 ± 2,00055 ± 71.00 ± 0.02ND
II140 ± 789,200 ± 20066 ± 141.89 ± 0.21ND
III160 ± 4412,000 ± 70075 ± 46.25 ± 0.78ND
hAb823 ± 116,700 ± 1,900290 ± 83I15 ± 1014,000 ± 4,500940 ± 3001.00 ± 0.08ND
II4 ± 37,300 ± 2,0001,800 ± 5002.70 ± 0.22ND
III27 ± 1412,000 ± 1,600450 ± 594.19 ± 0.37ND
LWHI2,100 ± 1001,500 ± 5000.7 ± 0.2I1,200 ± 20015,000 ± 5,00012.5 ± 4.21.00 ± 0.021.00 ± 0.06
II1,100 ± 20011,600 ± 2,50010.5 ± 2.32.11 ± 0.302.04 ± 0.27
III790 ± 2609,900 ± 1,50023.1 ± 1.96.95 ± 0.473.61 ± 0.37
YEGFP3,000 ± 14511,300 ± 1,9003.8 ± 0.6
  • a Triplicate cultures were analyzed for each condition, and all secretion and intracellular levels were determined by Western blotting. Values represent means±standard deviations.

  • b Relative fluorescence yields were normalized to the type I fusion protein for each species. ND, not determined.