TABLE 1.

Quantification by HAdV and JCPyV QPCR and presence of HEV evaluated by nested RT-PCR in different types of wastewater matrices obtained from a wastewater treatment plant

Sample typeDate of collection (day/mo/yr)Genome copies of:Presence of HEV
HAdV (assay 1)HAdV (assay 2)JCPyV
Sewage23/11/20041.16 × 105/ml1.40 × 104/ml7.99 × 103/ml
29/11/20049.54 × 104/ml3.12 × 104/ml6.76 × 103/ml+
21/12/20041.58 × 104/ml8.59 × 103/ml8.39 × 103/ml+
07/02/20054.76 × 102/mlNTa2.50 × 103/ml+
07/03/20052.56 × 103/mlNT8.92 × 103/mlNT
11/04/20052.07 × 103/mlNT1.15 × 103/ml+
Effluent23/11/20053.25 × 103/liter1.16 × 103/liter4.21 × 102/liter
29/11/20049.03 × 103/liter7.80 × 103/liter9.69 × 102/literNT
21/12/20041.78 × 103/liter5.94 × 102/liter2.33 × 102/liter
Sludgeb21/11/20045.06 × 103/ml4.33 × 103/ml3.95 × 104/mlNT
29/11/20041.26 × 103/ml3.94 × 101/ml6.00 × 101/mlNT
21/12/20048.05 × 102/ml1.54 × 102/ml6.90 × 102/ml
25/01/20056.49 × 103/ml5.30 × 103/ml1.07 × 102/mlNT
25/01/20051.04 × 104/ml2.29 × 102/ml2.80 × 101/mlNT
07/02/20051.11 × 104/ml7.73 × 102/ml2.40 × 101/mlNT
07/02/20054.97 × 102/ml3.67 × 101/ml6.02 × 103/mlNT
01/06/20041.67 × 103/ml7.25 × 102/ml4.54 × 101/mlNT
Biosolidc23/11/20044.34 × 105/gNTNT
29/11/20042.45 × 103/g2.43 × 102/g4.24 × 103/g
25/01/20051.18 × 103/gl1.02 × 104/g7.71 × 103/g+
25/01/20052.17 × 103/g5.00 × 102/g4.39 × 103/gNT
07/02/20055.96 × 102/g4.05 × 102/g6.20 × 102/gNT
07/02/20052.02 × 103/g2.94 × 102/g3.42 × 102/g+
01/06/20042.37 × 103/g8.09 × 102/g2.03 × 103/gNT
  • a NT, not tested.

  • b Corresponding to 0.18 to 0.2 g of dry weight.

  • c Corresponding to 0.25 g of dry weight.