TABLE 2.

Observed and estimated richnesses of archaeal and bacterial amoA gene librariesa

SiteArchaeal amoABacterial amoAb
No. of clones sequencedNo. of OTUs (5%)Chao1 (5%)PCR amplification/no. of clones sequencedNo. of OTUs (5%)Chao1 (5%)
January
    23245+
    32445
    4309122467
    523612
    6321013
October
    23189
    3241421
    432883777
    52267+
    6321015
Total28242716199
  • a OTUs were defined as 5% difference in nucleic acid sequence alignment, and Chao1-estimated richness was calculated using DOTUR.

  • b For bacterial amoA, positive PCR amplification is indicated by a plus sign or the number of clones screened; unsuccessful amplification is indicated by a minus sign.