TABLE 1.

Secretion yields of unfused scFv/scTCRs and scFv/scTCR-GFP fusions

scFv/scTCR classSecretion level unfused (μg/liter)aFusion typeSecretion level fused (μg/liter)a
OX2650 ± 7I69 ± 44
II48 ± 34
III60 ± 44
LWHI2,100 ± 100I1,200 ± 200
II1,100 ± 200
III790 ± 260
hAb275 ± 6I41 ± 5
II35 ± 8
III51 ± 2
hAb34,800 ± 200I1,300 ± 500
II4,000 ± 300
III1,800 ± 100
hAb480 ± 16I40 ± 8
II20 ± 11
III55 ± 14
hAb781 ± 12I270 ± 100
II140 ± 78
III160 ± 44
hAb823 ± 11I15 ± 10
II4 ± 3
III27 ± 14
hAb1020 ± 8I36 ± 8
II13 ± 10
III20 ± 7
scFv A4,000 ± 1,100I1,500 ± 150
II2,900 ± 900
III2,400 ± 700
yEGFP3,000 ± 145NAbNA
  • a Triplicate cultures were analyzed for each construct, and values represent means±standard deviations. The final cell density was nearly the same for each construct, and therefore, comparison by volumetric yields is similar to comparison by per-cell yields.

  • b NA, not applicable.