TABLE 3.

Inhibition of activity of C. glutamicum wild-type AHAS by branched-chain amino acids

Presence of inhibitor (5 mM)Residual activity (%)a
ValineIsoleucineLeucineMeasuredbCalculatedc
+44
+61
+62
++4326.8
++4227.3
+++4316.7
  • a The activity without addition of any inhibitor is considered 100%.

  • b The values are averages of at least three independent measurements; the standard deviations were within ± 7%.

  • c Calculated for cumulative inhibition. This activity was calculated as follows: activity (percent) = [1−(percent inhibition with valine/100)] × [1−(percent inhibition with leucine/100)] × [1−(percent inhibition with isoleucine/100)] × 100.