TABLE 1.

Production of ECC by E. coli O157:H7 strains 43895-EPS, ATCC 43895-, and 43895+ on a per-cell biomass basis as affected by growth medium and temperature

MediumStrainAmt of ECC (μg/ml)a at:
22°C12°C
HLJA43895-EPSa 1,402 ba 2,676 a
ATCC 43895-b 103 ab 152 a
ATCC 43895+b 83 bb 136 a
mTSA43895-EPSa 135 ba 289 a
ATCC 43895-b 66 ab 55 a
ATCC 43895+c 39 bb 121 a
  • a Amount of ECC produced on a per-cell biomass basis. A standard initial population (104 CFU) of E. coli O157:H7 was inoculated onto each plate and was incubated at 22°C for 3 days or 12°C for 5 days. Values represent the amount of ECC present in suspension at an optical density at 750 nm of 1.0. Within a medium and temperature, values in a column that are not preceded by the same letter are significantly different (P ≤ 0.05). Values in the same row that are not followed by the same letter are significantly different (P ≤ 0.05).