TABLE 3.

Plasmids used in this study

PlasmidDescriptionUseSource
pMK4B. subtilis-E. coli shuttle vector; CmrTesting of transformation efficiencySullivan et al.a
pCR2.1-TOPOTopoisomerase I-activated cloning vector with 3′ overhangs; Kanr AmprSubcloning and construction of nonreplicable plasmids for insertion mutagenesisInvitrogen
pME3pCR2.1-TOPO containing a 0.7-kb putative NRPS fragmentThis study
pME11pCR2.1-TOPO containing a 0.7-kb ces PCR fragmentThis study
pMECpCR2.1-TOPO containing a 1.3-kb ces PCR fragmentInsertion mutagenesisThis study
  • a See reference 42.