TABLE 5.

Inhibition of AHAS activity by branched-chain amino acids in the C. glutamicum ΔilvN strain harboring pECKAilvBNC with mutations

Mutation on plasmidRelative activity (%) in presence of 10 mM amino acida
NoneValineIsoleucineLeucine
None100525765
M8100112112105
M11100326182
M131009510898
  • a The values are averages of at least three independent measurements; the standard deviations were within ± 18%.