TABLE 1.

Chromatographic enrichmenta of 5′His6WS/DGAT from the soluble protein fraction of E. coli Rosetta(DE3)pLysS (pET23a::5′His6atf)

Purification stepTotal protein (mg)Specific enzyme activity (nmol mg of protein−1 min−1)Enrichment factor% Yield
Soluble fraction1,828.48.61100
SP-Sepharose concn182.619.52.322.6
Ni-NTA concn12.8165.219.213.4
  • a Values are averages of two independent measurements.