TABLE 2.

Specific enzyme activities of 5′His6WS/DGAT with alkanethiols as alternative acyl acceptors

Acyl acceptor% Activity with 1-hexadecanol as acyl acceptora
1-Hexadecanol100
1-Hexadecanethiol10.4
1,8-Octanedithiol59.3b
1-S-Monopalmitoyloctanedithiol13.4
  • a Activities were measured in radiometric assays with [1-14C]palmitoyl-CoA as the acyl donor by employing scintillation counting. Values are averages of two independent measurements.

  • b 1-S-Monopalmitoyloctanedithiol synthesized from 1,8-octanedithiol can also serve as the acyl acceptor, resulting in the synthesis of 1,8-S-dipalmitoyloctanedithiol (formation of 1-S-monopalmitoyloctanedithiol, 55.7%; formation of 1,8-S-dipalmitoyloctanedithiol, 3.6%).