TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant phenotype and/or genotypeSource or reference
Strains
    E. coli DH10BCloning strainGibco BRL
    E. coli GEc137(pGEc47ΔB)DH-1 thi fadR29
    P. putida GPo12(pGEc47ΔB)P. putida GPo1 cured of the OCT plasmid29
Plasmids
    pGEc47ΔBContains all genes necessary for growth on n-alkanes except an alkane hydroxylase gene29
    pCom8Broad-host-range expression vector with PalkB, Gmr oriT alkS31
    pCom8-PFR1500P450 operon in pCom8This study
    pCR2.1-TOPOPCR cloning vectorInvitrogen
    TOPO-HXN-1500pCR2.1-TOPO with HXN-1500 PCR fragmentThis study
    pKKPalkE. coli expression vector with PalkB, Apr31
    pZErO2.1Cloning vector, KmrInvitrogen
    pZErO2.1-Apa6.1pZErO2.1 with 6.1-kb ApaI fragmentThis study