Primers used in this study

PrimerSequence (5′→3′)Length (nt)aGC content (%)Tm (°C)bSpecificityReference
1406FTGY ACA CAC CGC CCG T1665.655.6Bacteria19
L189RTAC TGA GAT CYT TMA RTT C1934.249.1Bacteria36
27FAGA GTT TGA TCM TGG CTC AG2047.556.3Bacteria20
530FCTCC GTG CCA GCA GCC GCG G1978.967.5Bacteria20c
Hal1RCTC TAA AAG GAG GTA TTC1838.949.1SaprospiraceaeThis study
Hal3RAGC TAT GAG ACT TTG ACT1838.949.1SaprospiraceaeThis study
26FAGG ATG AAC GCT AGC GGG1861.158.2BacteriaThis study
HAL-1473RCTT AGC CCC AGT TAC TGG TTT T2245.558.4HAL subclusterThis study
LD2-1473RCTT AGC CCC AGT CAC TAG TTT T2245.558.4LD2 subclusterThis study
GKS-1473RCTT AGC CCC AGT CAT TGG TTT T2245.558.4GKS2-217 subclusterThis study
SAP-1490RCGG CTA CCT TGT TAC GAC TTA G225060.3Bacteria20c
  • a nt, nucleotides.

  • b Tm, melting temperature [Tm = 69.3 + 41 × [(nG + nC)/s] − (650/s), where nG is the number of G’s in the sequence, nC is the number of C’s in the sequence, and s is the length of the sequence].

  • c Primers were modified to amplify preferentially target bacterial sequences.