TABLE 2.

Genes expressed more highly under biofilm conditions after 8 h of growth

Type of proteinsN315 open reading frameBiofilm vs planktonic cells (fold difference)NameProductSDCV
Secreted proteinsSA20973.521Protein similar to secretory antigen precursor SsaA0.1173.31
SA20932.728 ssaA Secretory antigen precursor SsaA homolog0.2157.874
SA06202.534Secretory antigen SsaA homologue0.1254.913
Cell wall-associated proteinsSA246012.276 icaD Intercellular adhesion protein D0.2752.236
SA24598.238 icaA Intercellular adhesion protein A0.5146.237
SA24623.584 icaC Intercellular adhesion protein C0.38110.633
SA23563.12 isaA Immunodominant antigen A0.0983.154
SA24613.039 icaB Intercellular adhesion protein B0.2337.656
SA18932.604Lipoprotein precursor0.1826.999
Transporter proteinsSA05896.344Protein similar to ABC transporter ATP-binding protein0.1061.673
SA05315.629 prop Proline/betaine transporter homologue0.2945.222
SA21425.002Protein similar to multidrug resistance protein0.0611.221
SA22003.714Protein similar to ABC transporter ATP-binding subunit0.0812.169
SA11833.878 opuD Glycine betaine transporter0.0862.228
SA22013.447Protein, similar to ABC transporter permease protein0.0611.762
SA15193.367 aapA d-Serine/d-alanine/glycine transporter0.0822.443
SA06823.299Protein similar to di-tripeptide ABC transporter0.082.425
SA15472.98 ptaA Phosphotransferase system N-acetylglucosamine-specific II ABC component0.248.051
SA22022.7Protein similar to ABC transporter periplasmic amino acid-binding protein0.2258.327
SA08132.67 mnhA Na+/H+ antiporter subunit0.1184.434
SA16992.633Protein similar to transporter0.35113.339
SA07932.615 dltA d-Alanine-d-alanyl carrier protein ligase0.1033.937
SA21722.59 gltT Proton/sodium-glutamate symport protein0.1515.849
SA07332.575 secG Probable protein export membrane protein0.124.679
SA04932.52 secE Preprotein translocase subunit0.1485.868
Physiological proteinsSA09124.061 qoxB Quinol oxidase polypeptide I QoxB0.143.445
SA09133.513Protein similar to quinol oxidase polypeptide II QoxA0.1995.672
SA09113.229 qoxC Quinol oxidase polypeptide III QoxC0.1534.745
SA09103.218Protein similar to quinol oxidase polypeptide IV QoxD0.1083.353
SA05053.554 fus Translational elongation factor G0.2346.585
SA05023.337Protein similar to ribosomal protein0.1564.677
SA10753.284 hmrB HmrB protein0.0812.457
SA08423.107 FabH 3-Oxoacyl-(acyl carrier protein) synthase homologue0.1865.991
SA23542.956Protein similar to acyltransferase0.1354.552
SA09452.946 pdhC Dihydrolipoamide S-acetyltransferase component of pyruvate dehydrogenase complex E20.1986.734
SA07312.874 eno Enolase0.1685.836
SA22042.862Phosphoglycerate mutase pgm homolog0.2117.38
SA17172.861Glutamyl-tRNAGln amidotransferase subunit C0.2729.502
SA21912.833Protein similar to NirC0.2157.605
SA08022.832Protein similar to NADH dehydrogenase0.29610.437
SA08432.621 fab 3-Oxoacylsynthase0.166.093
SA20772.614Protein similar to biotin biosynthesis protein0.1937.402
SA20272.557 adk Adenylate kinase0.29311.443
SA15482.516Protein similar to acylglycerol-3-phosphate O-acyltransferase homolog0.1345.332
Ribosomal proteinsSA20163.324 rpsI 30S ribosomal protein S90.1283.856
SA20173.241 rplM 50S ribosomal protein L130.1113.43
SA14713.173 rpmA 50S ribosomal protein L270.1424.466
SA20303.109 rpmD 50S ribosomal protein L300.154.818
SA20453.058 rplW 50S ribosomal protein L230.2247.318
SA10813.039 rpsP 30S ribosomal protein S160.0792.605
SA20292.874 rplO 50S ribosomal protein L150.1625.653
SA05042.836 rpsG 30S ribosomal protein S70.2458.648
SA04952.834 rpsK 50S ribosomal protein L110.2629.254
SA14732.79 rplU 50S ribosomal protein L210.2237.978
SA03542.772 rpsR 30S ribosomal protein S180.0712.557
SA05032.757 rpsL 30S ribosomal protein S120.1916.919
SA20312.75 rpsE 30S ribosomal protein S50.2719.837
SA04972.737 rplJ 50S ribosomal protein L100.1364.973
SA11162.736 rpsO 30S ribosomal protein S150.0853.097
SA20322.662 rplR 50S ribosomal protein L180.2489.305
SA04962.629 rplA 50S ribosomal protein L10.1475.605
SA20482.617 rpsJ 30S ribosomal protein S100.2057.846
SA20352.611 rplE 50S ribosomal protein L50.30211.562
SAS0792.587 rpsN 30S ribosomal protein S140.2449.43
Other proteinsSA21065.192Protein similar to protein of pXO2-460.2715.22
SA19494.484Lytic regulatory protein truncated with Tn5540.1773.942
SA19563.783Lytic regulatory protein truncated with Tn5540.1393.681
SA04693.215 ftsH Cell division protein0.1193.712
SA10233.174 ftsL Cell division protein0.0250.796
SA03532.964 ssb Single-strand DNA-binding protein of phage φPVL0.1916.461
SA13052.77 hu DNA-binding protein II0.1354.89
Hypothetical proteinsSA02717.686Conserved protein0.1041.347
SA02924.76Conserved protein0.1733.639
SA06093.935Conserved protein0.1954.945
SA08903.847Conserved protein0.2897.516
SA14033.677Conserved protein0.1423.868
SA05883.593Conserved protein0.1323.67
SA10533.231Conserved protein0.1063.272
SA14723.155Conserved protein0.1735.497
SA14193.084Conserved protein0.1254.068
SA21333.001Conserved protein0.2488.261
SA19442.909Hypothetical protein0.2458.414
SA10562.819Hypothetical protein0.2629.292
SA04122.815Conserved protein0.1164.121
SA14022.805Conserved protein0.1174.164
SA02912.802Hypothetical protein0.0682.412
SA19712.762Hypothetical protein0.1615.841
SAS0482.658Hypothetical protein0.176.389
SA23782.643Conserved protein0.1495.635
SA21432.629Conserved protein0.166.079
SA12932.612Conserved protein0.2198.378
SA19122.511Hypothetical protein0.0692.742
SA09752.502Conserved protein0.25310.124