Two-way ANOVA used to test for differences in BCC between the dam, middle, and river incubations throughout all experimental variants and different time intervalsa

ProbeBottom-up manipulationb (F value)Top-down manipulationc (F value)
24 h48 h72 h96 h24 h48 h72 h96 h
  • a We tested the differences in the relative contributions (as a percentage of total DAPI-stained bacteria) of five different phylogenetic groups of bacteria targeted by the probes BET42a, R-BT065, GAM42a, CF319a, and HGC69a induced by transplanting the samples from the dam area upstream to the middle and river areas and by manipulating top-down control via size fractionation of water samples. The significant differences are in boldface type. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

  • b Dam samples transplanted to the middle and river parts.

  • c Effects of distinct grazing pressures when bacterivore-free treatments are compared to bacterivore-enhanced treatments.