TABLE 3.

Genes expressed more highly under biofilm conditions after 16 h of growth

Type of proteinsN315 open reading frameBiofilm vs planktonic cells (fold difference)NameProductSDCV
Cell wall-associated proteinsSA24238.485 clfB Clumping factor B0.0991.167
SA05197.747 sdrC Ser-Asp-rich fibrinogen-binding bone sialoprotein-binding protein0.1572.027
SA07424.81 clfA Fibrinogen-binding protein A clumping factor0.1893.931
SA05874.297Lipoprotein streptococcal adhesin PsaA homologue0.2796.501
SA18932.589Lipoprotein precursor0.0963.717
Transporter proteinsSA058911.177Protein similar to ABC transporter ATP-binding protein0.1781.592
SA15192.899 aapA d-Serine/d-alanine/glycine transporter0.0571.958
SA22032.813Protein similar to multidrug resistance protein0.30810.95
SA09282.577Protein similar to cation ABC transporter0.29111.285
SA24262.555 arcD Arginine/ornithine antiporter0.0773.027
Physiological proteinsSA22048.233Phosphoglycerate mutase pgm homolog0.0480.585
SA02198.033 pflA Formate-acetyltransferase-activating enzyme0.2192.728
SA02187.14 pflB Formate acetyltransferase0.1822.555
SA24256.033 arcC Carbamate kinase0.3435.678
SA24285.122 arcA Arginine deiminase0.3486.798
SA02254.386Protein similar to glutaryl-coenzyme A dehydrogenase0.3066.967
SA09944.293 sdhC Succinate dehydrogenase cytochrome b-5580.1854.303
SA02324.067 lctC l-Lactate dehydrogenase0.2165.311
SA15533.258 fhs Formyltetrahydrofolate synthetase0.1955.974
SA09133.195Protein similar to quinol oxidase polypeptide II QoxA0.1334.157
SA15313.146 ald Alanine dehydrogenase0.1825.776
SA24273.088 arcB Ornithine transcarbamoylase0.1825.878
SA01333.079 dra Deoxyribose-phosphate aldolase0.144.543
SA19062.993 atpG ATP synthase gamma chain0.1735.767
SA19392.988Deoxyribose phosphate aldolase0.0581.945
SA20082.924 alsS Alpha-acetolactate synthase0.2348.015
SA09112.889 qoxC Quinol oxidase polypeptide III QoxC0.0381.307
SA19102.823 atpE ATP synthase C chain0.2288.065
SA02312.822Protein similar to flavohemoprotein0.1394.942
SA10882.794 sucC Succinyl-coenzyme A synthetase0.238.221
SA09122.781 qoxB Quinol oxidase polypeptide I QoxB0.0632.268
SA12452.741 odhA 2-Oxoglutarate dehydrogenase E10.1525.53
SA01712.651 fdh NAD-dependent formate dehydrogenase0.0893.358
SA19112.613 atpB ATP synthase A chain0.1094.186
SA15612.575 murC UDP-N-acerylmuramate-alanine ligase0.0762.961
Ribosomal proteinSA20352.504 rplE 50S ribosomal protein L50.1385.518
Other proteinsSA19845.321 asp23 Alkaline shock protein 23 (ASP23)0.0961.805
SA04522.612 veg VEG protein homologue0.0471.799
SA19492.595Lytic regulatory protein truncated with Tn5540.0873.357
Hypothetical proteinsSA22688.134Hypothetical protein0.2693.302
SA05886.614Conserved protein0.3224.876
SA19855.151Hypothetical protein0.132.516
SA02714.887Conserved protein0.1232.519
SA19864.65Hypothetical protein0.2385.121
SA02273.765Conserved protein0.0912.418
SA09293.547Conserved protein0.1955.507
SA14033.155Conserved protein0.2297.248
SA04122.991Conserved protein0.0541.793
SA21332.945Conserved protein0.1826.177
SA19122.771Hypothetical protein0.1977.125
SA03712.707Hypothetical protein0.2318.524
SA19252.702Conserved protein0.1124.138
SA14022.69Conserved protein0.0562.068
SA02922.58Hypothetical protein0.1315.084
SA19162.532Conserved protein0.124.745
SA06092.5Conserved protein0.0592.378