TABLE 1.

Purification of LNBP from B. bifidum JCM 1254

Purification stepTotal protein (mg)Total activity (U)Sp act (U/mg)Purification (n-fold)
Crude extract524370.071
30% saturated (NH4)2SO4181220.121.8
Butyl-Toyopearl3.41.90.567.8
DEAE-Toyopearl0.671.52.230
Mono-Q 1st0.231.04.664
Mono-Q 2nd0.0500.5010139