TABLE 1.

Dynamics and persistence of characteristic T-RFs and identification of the T-RFs

Position (bp)Ratio of T-RF area at indicated time after administrationaTrendbRatio of T-RF area in inoculum fecesIdentification by 16S clone libraries
FecesSmall intestineCecumColon
1 wk2 wk4 wk
560.17 ± 0.020.63 ± 0.08*2.72 ± 0.89*3.91 ± 1.09*1.50 ± 0.31*1.23 ± 0.34*0.32 ± 0.02NDc
1840.41 ± 0.070.70 ± 0.06*3.87 ± 1.81*3.24 ± 0.64*1.82 ± 0.58*1.84 ± 0.980.84 ± 0.10Uncultured Clostridium cluster XVIa
1960.60 ± 0.060.66 ± 0.060.91 ± 0.06*1.16 ± 0.17*0.89 ± 0.201.09 ± 0.27NDUncultured Clostridium cluster XVIa
231NDND0.2014.3 ± 11.61.29 ± 0.361.79 ± 0.75PNDUncultured Clostridium cluster VI
36620.7 ± 5.371.03 ± 0.08*1.07 ± 0.37*1.45 ± 0.60*1.08 ± 0.32*0.85 ± 0.16*0.64 ± 0.13Gammaproteobacteria”and Coriobacteriaceae
4400.31 ± 0.010.32 ± 0.050.24 ± 0.01*6.25 ± 1.17*2.62 ± 0.21*2.52 ± 0.54*PNDUncultured firmicutes
7300.33 ± 0.050.85 ± 0.170.52 ± 0.09ND0.91 ± 0.15*0.61 ± 0.171.37 ± 0.26Uncultured Clostridium clusters VI and IX
808NDNDND2.88 ± 1.251.59 ± 0.301.24 ± 0.32PNDND
  • a Values are ratios of a characteristic T-RF area to total area (mean ± standard error). Values for small intestine, cecum, and colon were determined at 8 weeks. *, Significantly different by paired t test from the value for the sample at 1 week.

  • b Changes in T-RF area after administration of human inoculum over time, ↑, increase; ↓, decrease; P, persistent in intestine.

  • c ND, not detected as T-RFs or clones.