TABLE 2.

Purification of the citrate synthase

Purification stepTotal protein (mg)Total act (U)Sp act (U/mg protein)Purification factorYield (%)
Crude extract508.1304.80.61.0100
Q-Sepharose20.272.73.66.024
MonoQ5.352.59.916.517
Affinity0.815.619.632.65