TABLE 1.

Strains and plasmids used in this study

Strain or plasmidGenotypeaSource or reference
Strains
    V. harveyi BB120Wild type63
    P. aeruginosa PAO1Wild type24
    E. coli K-12Wild typeATCC 25404
    E. coli K-12 MG1655F lambda ilvG rfb-50 rph-16, 43
    E. coli K-12 MG1655 motABΔmotAB uvrC::Tn1043
    E. coli K-12 MG1655 cysBΔcysB::Tn5Kan-229
    E. coli JM109recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) F′ [traD36 proAB+ lacIq lacZΔM15]71
    E. coli MC4100araD139 Δ(argF-lac) U169 rpsL150 relA1 deoC1 ptsF25 rbsR flbB530134
    E. coli MC4100 cysBaraD139 Δ(argF-lac) U169 rpsL150 relA1 deoC1 ptsF25 rbsR flbB5301 cysB34
    E. coli C600thi-1 thr-1 leuB6 lacY1 tonA21 supE44 rfbD134
    E. coli C600 cysBthi-1 thr-1 leuB6 lacY1 tonA21 supE44 rfbD1 cysB34
    E. coli EJ500Prototrophic, constitutive flagellar synthesis, selected from W3110 for motility (26)27
    E. coli JCB495MC1000 recD65
Plasmids
    R1drd19finO; Ampr Kmr Cmr Smr; IncFII (conjugation plasmid to promote biofilm formation)45
    pCM18Emr; pTRKL2-PCP25-RBSII-gfpmut3*-T0-T1 (GFP plasmid for visualizing biofilm)19
  • a Ampr, Kmr, Cmr, Smr, and Emr are ampicillin, kanamycin, chloramphenicol, streptomycin, and erythromycin resistance, respectively.