TABLE 2.

Similarity values for the closest relatives of 16S rRNA gene sequences retrieved from phytoplankton culturesa

Source cultureDGGE bandPhylogenetic groupClosest relative% SimilarityNo. of base positions comparedGenBank accession no.Association with toxic dinoflagellateb
Rhodomonas sp.Rho2ACFBBacteroidetes bacterium D2197.8267AY030100No
Rhodomonas sp.Rho2BRoseobacterMarine bacterium ATAM173a_17100.0247AF359532Yes
Rhodomonas sp.Rho2CCFBFlexibacter sp. strain D898.5269AF125323No
Emiliana huxleyiEmh3CCFBBacterium DG1030100.0294M62799Yes
Emiliana huxleyiEmh3FAlteromonadaceaeAlteromonas sp. strain JLN-A587100.0484X86461No
Phaeocystis cf. globosaPhc4aCFBUncultured marine bacterium OTU_A98.3292AF207850No
Phaeocystis cf. globosaPhc4CRoseobacterUncultured bacterium SB-8/30-AG100.0302AJ319843No
Skeletonema costatumSkc5ACFBUncultured marine bacterium OTU_A99.4311AF207850No
Skeletonema costatumSkc5BCFBUncultured Bacteroidetes bacterium98.5407AF466714No
Skeletonema costatumSkc5DSphingomonasErythrobacter aquimaris100.0329AY461443No
Skeletonema costatumSkc5GSphingomonasErythrobacter aquimaris99.4477AY461443No
Chaetoceros cf. tortissimusCht6BCFBUncultured bacterium clone ARKIA-13796.9288AF468277No
Chaetoceros cf. tortissimusCht6CAlteromonadaceaePseudoalteromonas sp. strain RE10F/2100.0329AF118018No
Chaetoceros cf. tortissimusCht6ESphingomonasErythrobacter aquimaris strain SW-14098.4246AY461443No
Thalassiosira gravidaThg7CCFBUncultured bacterium clone s3495.3428AY171371No
Thalassiosira gravidaThg7ERoseobacterSulfitobacter sp. strain HEL-77100.0301AJ534229No
Thalassiosira gravidaThg7GRhizobiumUncultured alpha-proteobacterium strain GWS-BW-H54M99.7288AY515417No
Pseudopedinella elasticaPse8ACFBUncultured Bay of Fundy bacterium BA10-1C98.6212AF323553No
Pseudopedinella elasticaPse8BRoseobacterMarine alpha-proteobacterium AS-2699.2425AJ391187No
Pseudopedinella elasticaPse8CAlteromonadaceaeUncultured gamma-proteobacterium97.9329AF466930No
Pseudopedinella elasticaPse8EMarinobacterMarinobacter sp. strain PCOB-2100.0338AJ000647Yes
Nannochloropsis granulataNc19CRoseobacterAlpha-proteobacterium DGGE band C201100.0267AF466928Yes
Nannochloropsis granulataNc19DRoseobacterRoseobacter sp. strain DG86999.2242AY258074Yes
Nannochloropsis granulataNc19ERoseobacterRoseobacter sp. strain DG869100.0248AY258074Yes
Micromonas pusillaMcp10BRoseobacterRoseobacter sp. strain KT0202a98.7253AF305498No
Micromonas pusillaMcp10CCFBBacterium DG89090.9452AY258122No
Micromonas pusillaMcp10DSphingomonasAlpha-proteobacterium MBIC395299.5380No
Tetraselmis sp.Trs11ARoseobacterRoseobacter sp. strain ANT9276a99.2257AY167262No
Tetraselmis sp.Trs11CRoseobacterUncultured bacterium clone OHKB4.3198.1209AB094841No
Tetraselmis sp.Trs11ERoseobacterUncultured Rhodobacter group clone D103100.0265AF367399No
Tetraselmis sp.Trs11FRoseobacterUncultured Rhodobacter group clone D10399.0391AF367399No
Prorocentrum minimumPrm12BAlteromonadaceaeUncultured bacterium CTD70B96.8282AF469317No
Prorocentrum minimumPrm12cRoseobacterRoseobacter sp. strain DG94299.3281AY258088Yes
Prorocentrum minimumPrm12DRoseobacterUncultured proteobacterium clone BMS898.3289AY193221No
Prorocentrum minimumPrm12FRoseobacterAntarctobacter sp. strain 667-12100.0261AJ294356Yes
Scrippsiella sp.Scp13ACFBCryomorphaceae bacterium UST2002080192.9297AB125062No
Scrippsiella sp.Scp13BCFBUncultured Cytophagales bacterium clone SL-6a96.5462AY337037No
Scrippsiella sp.Scp13DRoseobacterMarine bacterium ATAM173a_17100.0276AF359532Yes
Scrippsiella sp.Scp13FGamma-ProteobacteriaMarine gamma-proteobacterium HTCC2188100.0279AY386344No
A. fundyense CB301Isolate CB2CFBArctic sea ice-associated bacterium ARK1000793.0299AF468407No
A. fundyense CB301Isolate CB8RoseobacterMarine bacterium SCRIPPS_73299.8492AF359534No
A. fundyense CB301Isolate CB3MarinobacterMarinobacter sp. strain PCOB-2100.0520AJ000647Yes
A. fundyense CB301Afe14ERoseobacterAlpha-proteobacterium GMDJE10F198.8397AY162092No
A. fundyense 48.1Afa15ARoseobacterMarine bacterium SCRIPPS_732100.0269AF359534No
A. fundyense 48.1Afa15BRoseobacterMarine bacterium SCRIPPS_73299.8492AF359534No
A. fundyense 48.1Afa15DRoseobacterMarine bacterium SCRIPPS_73299.0409AF359534No
A. fundyense 48.1Afa15DRoseobacterMarine bacterium SCRIPPS_73299.6515AF359534No
A. fundyense 48.2Afb16ACFBTenacibaculum mesophilum96.3299AB032502No
A. fundyense 48.2Afb16BCFBUncultured Bacteroidetes bacterium90.7389AY274863No
A. fundyense 48.2Afb16CRoseobacterUncultured alpha-proteobacterium99.7350AF466897Yes
A. fundyense 48.2Afb16DRoseobacterMarine bacterium ATAM173a_1799.3300AF359532Yes
A. fundyense 48.2Afb16EGamma-ProteobacteriaUncultured gamma-proteobacterium clone SIMO-358100.046AF022407No
A. fundyense 48.2Afb16FRoseobacterSulfitobacter sp. strain H2499.7334AY277266Yes
A. fundyense 5.6Afc17ACFBBacteroidetes bacterium KMM 391297.5360AY243096No
A. fundyense 5.6Afc17BCFBBacteroidetes DGGE band AB-1100.0475AY353553No
A. fundyense 5.6Afc17CRoseobacterMarine bacterium ATAM173a_1699.2264AF359531Yes
A. fundyense 5.6Afc17HRhizobium-AgrobacteriumMesorhizobium sp. strain DG102399.7393AY258096Yes
A. fundyense 38.3Afd18ACFBUncultured marine eubacterium OTU B98.6347AF207851No
A. fundyense 38.3Afd18BRhizobium-AgrobacteriumUncultured alpha-proteobacterium clone A115-2098.1312AY323139No
A. fundyense 38.3Afd18CCFBAntarcticum aff. vesiculatum99.1220Y17133No
A. fundyense 38.3Afd18DCFBUncultured Bacteroidetes100.0307AY353555No
A. tamarense ATRU4/1Atb4′FNo close relativesUnidentified bacterial clone K2-S-587.3401AY344413No
A. tamarense OK905-7Atc5′ACFBBacterium DG945100.0427AY258123Yes
A. tamarense OK905-7Atc5′BRoseobacterRuegeria atlantica99.7400AF124521No
A. tamarense OK905-7Atc5′CRoseobacterRuegeria sp. strain TCg-999.4345AJ515042No
A. minutum GT-PortAtd6′ARoseobacterUncultured Roseobacter 253-1699.5206AJ294352Yes
A. minutum GT-PortAtd6′BRoseobacterUncultured Roseobacter 253-16100.0306AJ294352Yes
A. minutum GT-PortAtd6′DRoseobacterUncultured Roseobacter 253-16100.0398AJ294352Yes
A. tamarense AATL01Ate7′ACFBUncultured bacterium SB-29-AG99.7341AJ319850No
A. tamarense AATL01Ate7′CRoseobacterMarine bacterium ATAM407_56100.0300AF359535Yes
  • a Sequences that were related to chloroplast 16S rRNA genes are not included.

  • b Cases in which there was >99% similarity between our sequence and a sequence that originated from a toxic dinoflagellate culture or bloom were considered to have positive evidence for association with toxic dinoflagellates. In cases where a reference is not listed, evidence for an association with toxic dinoflagellates was based on information available from GenBank entries.