TABLE 4.

E. coli K-12 genes repressed by ursolic acida

Geneb no.Ursolic acidFuranoneAI-2Description
10 μg/ml30 μg/ml
ERPERPERPERP
Genes with known functions
    arsCb3503−1.50.04461.40.01432.30.2574−1.30.8110Enzyme, drug/analog sensitivity
    b2789b2789−1.90.0557−2.50.037711.80.7871−66.90.1484Putative d-glucarate permease (MFS family)
    cspFb1558−1.60.0033−1.10.35681.80.3239−8.60.2342Cold shock-like protein
    cspGb0990−2.50.0085−1.70.01662.50.00111.30.2950Homolog of Salmonella cold shock protein
    cysBb1275−1.70.0377−1.40.01751.10.68771.40.0013Positive transcriptional regulator for cysteine regulon
    cysDb2752−2.50.0009−2.50.02401.20.27331.70.1271Central intermediary metabolism: sulfur metabolism
    cysIb2763−1.50.0692−1.70.00021.00.70772.50.0002Central intermediary metabolism: sulfur metabolism
    cysJb2764−3.60.0147−3.30.00901.20.35072.50.0024Central intermediary metabolism: sulfur metabolism
    cysKb2414−3.60.0026−3.30.00791.00.26912.50.0002Amino acid biosynthesis: cysteine
    frvRb3897−5.40.0061−2.00.17541.70.52803.30.0004Putative frv operon regulatory protein
    gntU_1b3436−1.50.0257−1.40.04284.30.88822.00.1005Transport of small molecules: carbohydrates, organic acids, alcohols
    narHb1225−1.60.0022−1.40.02811.50.05212.00.0029Energy metabolism, carbon: anaerobic respiration
    pheMb1715−1.60.01061.00.76171.30.54321.00.9909Aminoacyl tRNA synthetases, tRNA modification
    phePb0576−1.50.0210−1.10.49851.20.63641.30.4968Transport of small molecules: amino acids, amines
    rimLb1427−1.50.02201.00.71851.30.5425−1.60.2914Enzyme, ribosomes: maturation and modification
    rmfb0953−1.50.00291.00.66171.40.8536−1.80.0964Factor, ribosomes: maturation and modification
    rpmIb1717−1.60.00721.00.7076−1.10.3271−1.10.7165Structural component, ribosomal proteins: synthesis, modification
    slpb3506−1.50.0059−1.60.00202.50.1058−6.50.0013Outer membrane constituents
    ugpBb3453−1.40.0446−1.50.02102.20.0098−2.70.0026Transport of small molecules: carbohydrates, organic acids, alcohols
    ybiKb0828−2.40.0007−2.20.00511.20.29791.70.0103Putative asparaginase
    yhaDb3124−1.60.0254−2.60.00912.20.8505−2.30.4040Glycerate kinase I
    yhaFb3126−1.50.0089−2.40.00231.20.2202−1.60.0043alpha-dehydro-beta-deoxy-d-glucarate aldolase
    yhaGb3128−2.00.0039−2.20.00771.10.4891−2.10.1735(d)-Galactarate dehydrogenase
Genes with unknown functions
    b0309b0309−1.70.0420−1.30.15502.60.732616.70.4347ORF, hypothetical protein
    b0484b0484−1.50.0435−1.10.42461.70.0298−1.40.1332Putative enzyme, not classified
    b0485b0485−1.80.0092−1.30.01861.80.1369−1.60.0503Putative enzyme, not classified
    b0829b0829−1.50.0315−1.50.08981.00.58291.30.0213Putative transport, not classified
    b1729b1729−5.60.0029−2.00.13301.50.57533.30.0020Putative enzyme, not classified
    b2379b2379−1.50.01141.00.32461.60.00721.00.5700Putative enzyme, not classified
    hdeAb3510−1.70.0075−1.40.00772.90.0010−13.20.0000ORF, hypothetical protein
    hdeBb3509−1.80.0006−1.40.01023.90.0002−17.80.0000ORF, hypothetical protein
    yeeDb2012−2.30.0253−1.40.22781.30.64842.50.0277ORF, hypothetical protein
    yeeEb2013−12.60.0058−2.00.18161.10.92393.30.0079Putative transport, not classified
    yjeBb4178−1.40.00511.00.83291.50.0369−1.70.0466ORF, hypothetical protein
    ybhGb0795−1.40.0134−1.40.00202.80.2822−1.40.5428Putative membrane, not classified
    yhaUb3127−1.90.0744−4.20.003018.90.8100−1.20.3398Putative transport protein
  • a For comparison, differential expression is indicated for the effect of AI-2 and 60 μg/ml furanone (49). The underlined ratios indicate the corresponding genes were significantly repressed by ursolic acid. The highlighted genes were repressed both by 10 and 30 μg/ml ursolic acid. ER, expression ratio.