TABLE 3.

Steady-state kinetics of the condensation of oxaloacetate and acetyl-coenzyme A

SubstrateNative enzymeRecombinant enzyme
Km (μM)kcat (s−1)kcat/Km (M−1 s−1)Km (μM)kcat (s−1)kcat/Km (M−1 s−1)
Acetyl-CoA14.18.35.89 × 10512.38.87.15 × 105
Oxaloacetate4.31.93 × 1068.11.09 × 106