TABLE 1.

Amount of biofilm formation in coculture between lactic acid bacteria and yeasts

S. cerevisiae strainAbsorbance of crystal violet ina:
MonocultureL. mesenteroides IAM 1064P. acidilactici IAM 1233L. paracasei subsp. paracasei IFO3533L. sakei IFO3541L. casei subsp. rhamnosus IFO3831L. helveticus B-1L. bulgaricus B-56L. casei L-14L. acidophilus L-54S. cremoris H-61
None0.0360.0480.0220.0200.0490.0400.0350.0240.0220.029
X2180-1A0.0440.0590.0710.0800.0700.1080.0790.0940.0700.0780.064
Kyokai-60.0280.0710.0770.0650.0530.0710.0510.0920.0660.0860.068
Kyokai-70.0550.0690.0740.0490.0480.0740.0720.0860.0860.0890.083
Kyokai-90.0390.0440.0470.0390.0630.0480.0720.0700.0510.0690.077
Kyokai-100.0770.0970.1000.0920.084 0.208 0.0960.0820.089 0.170 0.097
Kyokai-110.0330.107 0.149 0.0910.1130.1340.0850.0620.0900.1270.092
Kyokai-120.0370.1290.1000.0860.080 0.198 0.0950.0700.067 0.157 0.094
Kyokai-130.0480.124 0.151 0.0740.092 0.210 0.0950.1150.0930.0920.100
IAM 41250.0400.0350.0510.0570.0470.0380.0400.0430.0440.0420.035
IAM 41400.0350.0240.0310.0430.0440.0330.0380.0420.0480.0480.050
IAM 41780.0300.0250.0310.0390.0380.0310.0360.0260.0650.0460.041
IAM 42740.0490.0630.0520.0660.0600.0690.0560.0380.0530.0740.038
IAM 49540.0280.0220.0390.0460.0430.0280.0380.0530.0400.0350.053
  • a Numerals given in the table represent absorbance of crystal violet extracted from crystal violet-stained biofilms. Values in boldface represent combinations with distinct biofilm formation. Results are shown for the species Leuconostoc mesenteroides, Pediococcus acidilactici, Lactobacillus paracasei, Lactobacillus sakei, Lactobacillus casei, Lactobacillus helveticus, Lactobacillus bulgaricus, Lactobacillus acidophilus, and Streptococcus cremoris.