TABLE 2.

Oligonucleotide primers and probes

Target geneSequence (5′ to 3′)aLocation in cluster (nucleotides)Amplicon size (bp)Accession no. or source
Singleplex and multiplex PCR
    O45 wzx1(F) CCG GGT TTC GAT TTG TGA AGG TTG7769-8295527This study
(R) CAC AAC AGC CAC TAC TAG GCA GAA
    O45 wzx2(F) TAT GAC AGG CAC ATG GAT CTG TGG7347-7601255
(R) TTG AGA CGA GCC TGG CTT TGA TAC
    O45 wzy1(F) GAA ATT ATG CCA TCT TGG CGA GCG9570-10066497This study
(R) CAT GTG AAG CCT GAA GGC AAA CTC
    O45 wzy2(F) CTG ATG TCA GGC CTC GTG GAA ATA9858-10308451
(R) ATG TAA CCA CAA TAA GGG AGC CCG
    O55 wzx1(F) AAT GGA ACA TTG CAA CAG CA10746-10895150AF461121
(R) TGT GGA TTC CAG AAA AGC AA
    O55 wzx2(F) TCT TGT AAC TAA GTG GCC ACA GGC10823-11505683
(R) ATA ACA CCC AAC CTA TAC CTC CCG
    O55 wzy1(F) GTG GTT TTG ACG ACT CGC TT9380-9526147AF461121
(R) CCA AAA AGC CCT GCA ACT AA
Real-time PCR
    O45 wzx(F) CGT TGT GCA TGG TGG CAT7472-754372This study
(R) TGG CCA AAC CAA CTA TGA ACT G
    O45 wzy(F) GGT GCT TTG TGA TAA TTC CTG ATG9616-969176This study
(R) TTA TAG CCG CCC CTA AAT TGC
    O45 wzx probe6-FAM d(ATT TTT TGC TGC AAG TGG GCT GTC CA)BHQ-17494-7519This study
    O45 wzy probe6-FAM d(TTG CTG CTG GCG GGA TAC CAA TGA T)BHQ-19643-9667This study
    O55 wzx(F) AAT TAA CGA ACA TAA CAC CCA ACC11416-11516101AF461121
(R) ATA TCT CTT CGT TAC TGT GTG TAT TTC
    O55 wzy(F) AGC TTT CCT GGC GGG TTT10118-1020487AF461121
(R) GCA CCA CGC TAT CTT TTT TCT TAA T
    O55 wzx probe6-FAM d(ACC TCC CGC TAA AAC CCC AAC TCT AGT AG)BHQ-111461-11489AF461121
    O55 wzy probe6-FAM d(CCG CGG CGA TAT TGG GTA CTG C)BHQ-110142-10163
  • a F, forward; R, reverse.