TABLE 1.

Specificity of the primer-probe combination

Microorganism or prepnStrainaCtb
Lactobacillus thermotoleranscJCM 11425T18.57 (0.09)
Negative controls
    Lactobacillus salivarius subsp. saliciniusJCM 104434.87 (0.22)
    Lactobacillus aviarius subsp. araffinosusJCM 5667T34.56 (0.77)
    Lactobacillus aviarius subsp. aviariusJCM 5666T34.34 (1.46)
    Lactobacillus crispatusJCM 581034.45 (0.08)
    Lactobacillus gallinarumJCM 2011T34.61 (0.23)
    Lactobacillus amylovorusJCM 1126T35.28 (0.25)
    Lactobacillus casei subsp. caseiJCM 1134T34.76 (0.81)
    Lactobacillus helveticusJCM 1120T32.27 (0.20)
    Lactobacillus mucosaeDSM 13345T35.31 (0.54)
    Lactobacillus fermentumJCM 1173T33.77 (0.47)
    Lactobacillus acidophilusJCM 1132T34.57 (0.76)
    Streptococcus equinusJCM 787635.41 (0.80)
    Lactococcus lactis subsp. lactisJCM 115835.70 (0.41)
    Enterococcus cecorumJCM 8724T34.94 (0.35)
    Pediococcus dextrinicusJCM 5887T34.83 (0.32)
    Leuconostoc mesenteroides subsp. mesenteroidesJCM 156434.46 (0.86)
    Clostridium coccoidesJCM 1395T34.43 (0.26)
    Clostridium butyricumJCM 1391T35.91 (0.46)
    Clostridium perfringensJCM 381736.34 (0.43)
    Bacillus coagulansJCM 2257T36.11 (0.19)
SDWd34.50 (0.48)
  • a Strains were obtained from the Japan Collection of Microorganisms (JCM) and the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSM).

  • b The values in parentheses are standard deviations.

  • c L. thermotolerans was the target organism.

  • d Nontemplate control.