TABLE 1.

Genes in the O-antigen gene cluster of E. coli serogroup O45

ORF no.Proposed gene nameLocation (nucleotides)No. of amino acids in gene productPutative functionMost significant homolog(s) (accession no.)% Identity/% similarity
1rmlB59-1198379dTDP-d-glucose-4,6-dehydrataseRmlB, dTDP-glucose 4,6-dehydratase, Salmonella enterica (AAG09513)83/87
2wbhP1246-2196316NAD-dependent epimerase/dehydrataseORF_12; similar to NAD-dependent epimerase/dehydratase family, Pseudomonas aeruginosa (AAM27579)42/58
3wbhQ2196-3230344GlycosyltransferaseProbable N-acetylgalatosaminyl transferase, trsF (S51265), and WbcO, Yersinia enterocolitica (CAA87703)50/68
4wbhR3231-3791186AcetyltransferaseAcetyltransferase, Oceanobacillus iheyensis (NP_693813) (BAC14847)37/58
5wbhS3830-5716628TrsG protein homolog, role in galactose modificationTrsG protein homolog (T44517), ORF9P (BAA85014), and WbgZ (AAG17416), Plesiomonas shigelloides61/79
6rmlC5797-6336179dTDP-4-dehydrorhamnose-3,5-epimerasePutative dTDP-4-dehydrorhamnose-3,5-epimerase, Aeromonas hydrophila (AAM22546)66/80
7wbhT6333-7151272dTDP-glucose-4,6-dehydratasePutative dTDP-glucose-4,6-dehydratase, Aeromonas hydrophila (AAM22547)55/69
8wzx7144-8403419O-antigen flippaseMembrane protein involved in the export of O-antigen and teichoic acid, 12 transmembrane domains, Burkholderia fungorum (ZP_00278649)25/44
9wbhU8387-9352321GlycosyltransferasePutative glycosyltransferase, Pyrococcus furiosus (NP_579088)29/49
10wzy9366-10514382O-antigen polymeraseTransmembrane protein, 9 transmembrane domains, Ralstonia solanacearum (NP_519421.1)27/45
11wbhV10507-11043178Serine acetyltransferasePutative acetyltransferase, Clostridium thermocellum (ZP_00313397.1)37/60
12wbhW11045-12055336UnknownEps11, Streptococcus thermophilus (AAN63793)30/49
13rmlA12102-12974290Glucose-1-phosphate thymidylyltransferaseRmlA, Raoultella terrigena (AAQ82933.1)78/89