TABLE 5.

GC-MS analysis of P. putida CA-3 mini-Tn5 mutants

P. putida CA-3Growth substrate (mM)PHA (% CDW)
Wild typePA (10)23.2
E28G12aPA (10)0
E22B11aPA (10)0
  • a E28G12 and E22B11 are both PhaC1 synthase-negative mutants. Cells were grown on PA for 48 h on E2 medium-8 mM NH4+ before assessment for PHA accumulation.