TABLE 2.

GC-MS analysis of P. putida CA-3 mini-Tn5 mutantsa

P. putida CA-3Growth in NH4+ concentration of:
00.2 mM0.4 mM
Wild type2.3 ± 0.99.35 ± 1.755.5 ± 0.5
D8D4000
E11D63.45 ± 0.755.75 ± 0.251.8 ± 0.8
  • a E11D6, citrate synthase-negative mutant; D8D4, malate dehydrogenase mutant. All cells were grown on 10 mM citrate (8 mM NH4+) for 18 h and resuspended in 10 mM PA for 36 h with 0, 0.2, and 0.4 mM NH4+, respectively, before PHA (% CDW) analysis.