TABLE 3.

PCR conditions used for the different assays

PCR gene(s)Primer or primer pairTemp (°C) [(time in s)]Amplicon size(s) (bp)
AnnealingExtension
Singleplex PCR
    O45 wzxO45wzx1, O45wzx259 (0)72 (30)527, 255
    O45 wzyO45wzy1, O45wzy259 (0)72 (30)497, 451
    O55 wzxO55wzx1, O55wzx250 (0)72 (8)150, 683
    O55 wzyO55wzy150 (0)72 (8)147
Multiplex PCR
    O45 (wzx and wzy)O45wzx2, O45wzy259 (0)72 (30)255, 451 (Fig. 1A)
    O55 (wzx and wzy)O55wzx2, O55wzy155 (0)72 (32)683, 147 (Fig. 1B)
    O45 (wzx) and O55 (wzx)O45wzx2, O55wzx255 (0)72 (32)255, 683 (not shown)